Beta-adrenergic agonists increase lung liquid clearance in anesthetized sheep.

We did experiments to determine whether beta-adrenergic agonists increase lung liquid clearance in anesthetized ventilated adult sheep and, if so, whether the increase is mediated by beta receptors and what mechanism is involved. We instilled 100 ml of autologous serum either alone or with a beta-adrenergic agonist (terbutaline, 10(-5) M, or epinephrine, 5.5 X 10(-6) M) into one lower lobe. After 4 h both terbutaline and epinephrine increased lung liquid clearance. The increase in lung liquid clearance was inhibited when propranolol (a beta blocker) or amiloride (a sodium channel blocker) was added to the terbutaline. Increased clearance was not explained by changes in pulmonary hemodynamics, pulmonary blood flow, or lung lymph flow. We conclude that beta-adrenergic agonists increase lung liquid clearance in anesthetized intact adult sheep. This increase is mediated through beta receptors and probably depends on increased active transport of sodium across the alveolar barrier.

[1]  B. Goodman,et al.  Effects of amiloride on sodium flux in isolated rat lungs , 1986 .

[2]  E. Crandall,et al.  Effects of terbutaline on sodium transport in isolated perfused rat lung. , 1986, Journal of applied physiology.

[3]  P. Barnes,et al.  Autoradiographic visualization of beta-adrenoceptor subtypes in human lung. , 1985, The American review of respiratory disease.

[4]  M. Matthay,et al.  Long-term clearance of liquid and protein from the lungs of unanesthetized sheep. , 1985, Journal of applied physiology.

[5]  E. Egan,et al.  Validation of [57Co]cyanocobalamin as an extracellular fluid marker and measurement of albumin exclusion from the interstitium in the rabbit. , 1985, Microvascular research.

[6]  J. Caldwell,et al.  Electrical currents flow out of domes formed by cultured epithelial cells , 1984, The Journal of cell biology.

[7]  E. Crandall,et al.  Regulation of transport across pulmonary alveolar epithelial cell monolayers. , 1984, Journal of applied physiology: respiratory, environmental and exercise physiology.

[8]  A. Jefferies,et al.  Effects of exercise on lung lymph flow in sheep and goats during normoxia and hypoxia. , 1984, The Journal of clinical investigation.

[9]  C. Cotton,et al.  Bioelectric properties and ion transport of airways excised from adult and fetal sheep. , 1983, Journal of applied physiology: respiratory, environmental and exercise physiology.

[10]  Staub Nc.  Alveolar flooding and clearance. , 1983 .

[11]  R. Olver.  Fluid balance across the fetal alveolar epithelium. , 1983, The American review of respiratory disease.

[12]  J. Widdicombe,et al.  Transepithelial transport by pulmonary alveolar type II cells in primary culture. , 1982, Proceedings of the National Academy of Sciences of the United States of America.

[13]  M. Matthay,et al.  Differential liquid and protein clearance from the alveoli of anesthetized sheep. , 1982, Journal of applied physiology: respiratory, environmental and exercise physiology.

[14]  N P Reddy,et al.  Intrinsic propulsive activity of thoracic duct perfused in anesthetized dogs. , 1981, Microvascular research.

[15]  M. Welsh,et al.  Ion transport by dog tracheal epithelium. , 1980, Federation proceedings.

[16]  D. E. Potts,et al.  Thyrotoxicosis: comparison of effects of thyroid ablation and beta-adrenergic blockade on metabolic rate and ventilatory control. , 1978, The Journal of clinical endocrinology and metabolism.

[17]  R. Nelson,et al.  Solute permeability of the alveolar epithelium in alloxan edema in dogs. , 1978, Journal of applied physiology: respiratory, environmental and exercise physiology.

[18]  D. Walters,et al.  The Role of Catecholamines in Lung Liquid Absorption at Birth , 1978, Pediatric Research.

[19]  I. Gessner,et al.  Solute permeability of the alveolar epithelium in acute hemodynamic pulmonary edema in dogs. , 1977, The American journal of physiology.

[20]  J I Hoffman,et al.  Blood flow measurements with radionuclide-labeled particles. , 1977, Progress in cardiovascular diseases.

[21]  K. Brigham,et al.  Preparation of chronic lung lymph fistulas in sheep. , 1975, The Journal of surgical research.

[22]  L. Strang,et al.  Ion fluxes across the pulmonary epithelium and the secretion of lung liquid in the foetal lamb , 1974, The Journal of physiology.

[23]  J. A. Johnson,et al.  Equilibrium and kinetic properties of the Evans blue-albumin system. , 1969, The American journal of physiology.

[24]  A. Taylor,et al.  Permeability of the Alveolar Membrane to Solutes , 1965, Circulation research.