Primary structure homology between the product of yeast cell division control gene CDC28 and vertebrate oncogenes

In the budding yeast, Saccharomyces cerevisiae, division is controlled in response to nutrient limitation1 and in preparation for conjugation2. Cells deprived of an essential nutrient or responding to mating pheromones cease division and become synchronous in the G1 interval, apparently constrained from completing a critical event. This event has been given the operational designation of ‘start’. We have isolated a large number of start mutations which confer on S. cerevisiae cells a conditional inability to complete start3,4 (Fig. 1) presumably because they define genes which must be expressed for the start event to be successfully completed. We have described the isolation on plasmids of one of the start genes, CDC28, by genetic complementation5 and initial characterization of its product6,7. We now describe the DNA sequence of the gene CDC28.

[1]  F. Sanger,et al.  DNA sequencing with chain-terminating inhibitors. , 1977, Proceedings of the National Academy of Sciences of the United States of America.

[2]  M. Kozak Comparison of initiation of protein synthesis in procaryotes, eucaryotes, and organelles. , 1983, Microbiological reviews.

[3]  S. Reed The selection of S. cerevisiae mutants defective in the start event of cell division. , 1980, Genetics.

[4]  S. Reed Preparation of product-specific antisera by gene fusion: antibodies specific for the product of the yeast cell-division-cycle gene CDC28. , 1982, Gene.

[5]  L. Hartwell,et al.  Genetic control of the cell division cycle in yeast. , 1974, Science.

[6]  Russell F. Doolittle,et al.  Nucleotide sequence and formation of the transforming gene of a mouse sarcoma virus , 1981, Nature.

[7]  T. Hunter,et al.  The transforming protein of Moloney murine sarcoma virus is a soluble cytoplasmic protein , 1983, Cell.

[8]  P. Nurse,et al.  Functionally homologous cell cycle control genes in budding and fission yeast , 1982, Nature.

[9]  R. D. Wade,et al.  Complete amino acid sequence of the catalytic subunit of bovine cardiac muscle cyclic AMP-dependent protein kinase. , 1981, Proceedings of the National Academy of Sciences of the United States of America.

[10]  S. Reed,et al.  Preliminary characterization of the transcriptional and translational products of the Saccharomyces cerevisiae cell division cycle gene CDC28 , 1982, Molecular and cellular biology.

[11]  G C Johnston,et al.  Coordination of growth with cell division in the yeast Saccharomyces cerevisiae. , 1977, Experimental cell research.

[12]  M. O. Dayhoff,et al.  Viral src gene products are related to the catalytic chain of mammalian cAMP-dependent protein kinase. , 1982, Proceedings of the National Academy of Sciences of the United States of America.

[13]  P. Sharp,et al.  Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids , 1977, Cell.

[14]  K. Nasmyth,et al.  Isolation of genes by complementation in yeast: molecular cloning of a cell-cycle gene. , 1980, Proceedings of the National Academy of Sciences of the United States of America.

[15]  P. Duesberg Retroviral transforming genes in normal cells? , 1983, Nature.

[16]  L. Hartwell,et al.  Reversible arrest of haploid yeast cells in the initiation of DNA synthesis by a diffusible sex factor. , 1973, Experimental cell research.

[17]  J Messing,et al.  A system for shotgun DNA sequencing. , 1981, Nucleic acids research.

[18]  R. Doolittle Similar amino acid sequences: chance or common ancestry? , 1981, Science.