DNAzyme-Functionalized R-Phycoerythrin as a Cost-Effective and Environment-Friendly Fluorescent Biosensor for Aqueous Pb2+ Detection

The sensitive detection of Pb2+ is of significant importance for food safety, environmental monitoring, and human health care. To this end, a novel fluorescent biosensor, DNAzyme-functionalized R-phycoerythrin (DNAzyme-R-PE), was presented for Pb2+ analysis. The biosensor was prepared via the immobilization of Iowa Black® FQ-modified DNAzyme–substrate complex onto the surface of SPDP-functionalized R-PE. The biosensor produced a minimal fluorescence signal in the absence of Pb2+. However, Pb2+ recognition can induce the cleavage of substrate, resulting in a fluorescence restoration of R-PE. The fluorescence changes were used to measure sensitively Pb2+ and the limit of detection was 0.16 nM with a linear range from 0.5–75 nM. Furthermore, the proposed biosensor showed excellent selectivity towards Pb2+ even in the presence of other metal ions interferences and was demonstrated to successfully determine Pb2+ in spiked lake water samples.

[1]  Liguang Xu,et al.  Ultrasensitive detection of lead ions based on a DNA-labelled DNAzyme sensor , 2015 .

[2]  Kevin W Plaxco,et al.  Electrochemical detection of parts-per-billion lead via an electrode-bound DNAzyme assembly. , 2007, Journal of the American Chemical Society.

[3]  P. Hsu,et al.  Antioxidant nutrients and lead toxicity. , 2002, Toxicology.

[4]  A. Cocherie,et al.  Direct measurement of lead isotope ratios in low concentration environmental samples by MC-ICP-MS and multi-ion counting , 2007 .

[5]  Mohammad Ramezani,et al.  Recent nucleic acid based biosensors for Pb2+ detection , 2017 .

[6]  A. Islam,et al.  Characterization and Application of 1-(2-Pyridylazo)-2-naphthol Functionalized Amberlite XAD-4 for Preconcentration of Trace Metal Ions in Real Matrices , 2010 .

[7]  Yi Lu,et al.  Improving fluorescent DNAzyme biosensors by combining inter- and intramolecular quenchers. , 2003, Analytical chemistry.

[8]  Yi Lu,et al.  A highly selective lead sensor based on a classic lead DNAzyme. , 2010, Chemical communications.

[9]  R. Thangam,et al.  Isolation and characterization of macromolecular protein R-Phycoerythrin from Portieria hornemannii. , 2013, International journal of biological macromolecules.

[10]  Jing Li,et al.  A highly sensitive and selective catalytic DNA biosensor for lead ions [9] , 2000 .

[11]  New transition-metal-dependent DNAzymes as efficient endonucleases and as selective metal biosensors. , 2003, Chemistry.

[12]  Yi Lu,et al.  Immobilization of a catalytic DNA molecular beacon on Au for Pb(II) detection. , 2005, Analytical chemistry.

[13]  M. Levy,et al.  Proximity ligation assays with peptide conjugate ‘burrs’ for the sensitive detection of spores , 2005, Nucleic acids research.

[14]  Runjhun Saran,et al.  A Silver DNAzyme. , 2016, Analytical chemistry.

[15]  D. Carpenter,et al.  Understanding the human health effects of chemical mixtures. , 2002, Environmental health perspectives.

[16]  Werasak Surareungchai,et al.  Paper-based scanometric assay for lead ion detection using DNAzyme. , 2015, Analytica chimica acta.

[17]  Juewen Liu,et al.  Metal Sensing by DNA. , 2017, Chemical reviews.

[18]  Freddy C. Adams,et al.  Determination of cadmium, copper and lead in environmental samples. An evaluation of flow injection on-line sorbent extraction for flame atomic absorption spectrometry , 1994 .

[19]  Xinhui Liu,et al.  DNAzyme-based biosensor for detection of lead ion: A review , 2017 .

[20]  J. Domingo,et al.  Concentrations of arsenic, cadmium, mercury, and lead in common foods and estimated daily intake by children, adolescents, adults, and seniors of Catalonia, Spain. , 2003, Journal of agricultural and food chemistry.

[21]  C. Niu,et al.  A Fluorescence Sensor for Lead (II) Ions Determination Based on Label-Free Gold Nanoparticles (GNPs)-DNAzyme Using Time-Gated Mode in Aqueous Solution , 2016, Journal of Fluorescence.

[22]  Shenshan Zhan,et al.  A mini-review on functional nucleic acids-based heavy metal ion detection. , 2016, Biosensors & bioelectronics.

[23]  Yingfu Li,et al.  DNA aptamer folding on gold nanoparticles: from colloid chemistry to biosensors. , 2008, Journal of the American Chemical Society.

[24]  Chunhai Fan,et al.  Metal ion-modulated graphene-DNAzyme interactions: design of a nanoprobe for fluorescent detection of lead(II) ions with high sensitivity, selectivity and tunable dynamic range. , 2011, Chemical communications.

[25]  Wenwan Zhong,et al.  Combing DNAzyme with single-walled carbon nanotubes for detection of Pb(II) in water. , 2011, The Analyst.

[26]  Guo-Li Shen,et al.  Graphene-DNAzyme based biosensor for amplified fluorescence "turn-on" detection of Pb2+ with a high selectivity. , 2011, Analytical chemistry.

[27]  Yi Lu,et al.  Biochemical and Biophysical Understanding of Metal Ion Selectivity of DNAzymes. , 2016, Inorganica chimica acta.

[28]  Maung Kyaw Khaing Oo,et al.  Highly sensitive multiplexed heavy metal detection using quantum-dot-labeled DNAzymes. , 2010, ACS nano.

[29]  Juewen Liu,et al.  Functional nucleic acid sensors. , 2009, Chemical reviews.

[30]  Hao Yan,et al.  Tunable Nanoscale Cages from Self-Assembling DNA and Protein Building Blocks. , 2019, ACS nano.

[31]  Lin Cui,et al.  Electrochemical Sensor for Lead Cation Sensitized with a DNA Functionalized Porphyrinic Metal-Organic Framework. , 2015, Analytical chemistry.

[32]  MONITORING OF HEAVY METALS BY ENERGY DISPERSIVE X-RAY FLUORESCENCE SPECTROMETRY* , 1988 .

[33]  P. J. Huang,et al.  Sensing parts-per-trillion Cd(2+), Hg(2+), and Pb(2+) collectively and individually using phosphorothioate DNAzymes. , 2014, Analytical chemistry.

[34]  Guangming Zeng,et al.  Current progress in biosensors for heavy metal ions based on DNAzymes/DNA molecules functionalized nanostructures: A review , 2016 .

[35]  Zhiyong Guo,et al.  A test strip for lead(II) based on gold nanoparticles multi-functionalized by DNAzyme and barcode DNA , 2015, Journal of Analytical Chemistry.

[36]  Itamar Willner,et al.  Amplified surface plasmon resonance and electrochemical detection of Pb2+ ions using the Pb2+-dependent DNAzyme and hemin/G-quadruplex as a label. , 2012, Analytical chemistry.

[37]  K. Y. Loh,et al.  DNAzyme sensors for detection of metal ions in the environment and imaging them in living cells. , 2017, Current opinion in biotechnology.

[38]  Hongwen Zhang,et al.  Fabrication of gold nanoparticles by laser ablation in liquid and their application for simultaneous electrochemical detection of Cd2+, Pb2+, Cu2+, Hg2+. , 2014, ACS applied materials & interfaces.

[39]  Hao Yan,et al.  Assembly of multienzyme complexes on DNA nanostructures , 2016, Nature Protocols.

[40]  J. Lakowicz,et al.  Single-molecule spectroscopic study of enhanced intrinsic phycoerythrin fluorescence on silver nanostructured surfaces. , 2008, Analytical Chemistry.

[41]  Lijia Liang,et al.  DNAzyme-based plasmonic nanomachine for ultrasensitive selective surface-enhanced Raman scattering detection of lead ions via a particle-on-a-film hot spot construction. , 2014, Analytical chemistry.

[42]  Bong Hyun Chung,et al.  Improving Pb2+ detection using DNAzyme-based fluorescence sensors by pairing fluorescence donors with gold nanoparticles. , 2011, Biosensors & bioelectronics.