Experimental Design and Analysis of the Optimal Fermentation Medium Based on Uniform Design and Support Vector Regression

Based on uniform design and support vector regression, UD-SVR, a novel experimental design and analysis method for the prescription optimization was proposed.It was applied to optimize the complicated fermentation medium including nine factors for a variant of Escherichia coli.The variant could produce glutamate decarboxylase which transformed gultamic acid into gamma aminobutyric acid in vitro.The optimization results of the medium by UD-SVR showed that OD630, an activity index of glutamate decarboxylase, increased from 1.528 in the initial median to 2.303 in the optimal medium after testing of 28 schemes.UD-SVR is more efficient than the reference models and has the potential to be widely used for experimental design and analysis of the prescription optimization.