DNA detection by surface enhanced resonance Raman scattering (SERRS).

This Education article outlines the different ways in which surface enhanced resonance Raman scattering (SERRS) can be used for the detection of DNA. The use of various different SERRS detection strategies that have allowed both sensitive and selective detection to be obtained is covered. Detection of DNA by SERRS involves the use of a dye with the DNA, whether as an intercalator or by direct covalent attachment. This generates strong SERRS signals that indicate the presence of the specific DNA sequence. The SERRS detection of DNA in different molecular biological assays is also discussed.

[1]  D. Whitcombe,et al.  Detection of PCR products using self-probing amplicons and fluorescence , 1999, Nature Biotechnology.

[2]  K. Rowlen,et al.  Thin silver films: influence of substrate and postdeposition treatment on morphology and optical properties , 1994 .

[3]  W. Smith,et al.  SERRS detection of PNA and DNA labelled with a specifically designed benzotriazole azo dye , 2001 .

[4]  D. Meisel,et al.  Adsorption and surface-enhanced Raman of dyes on silver and gold sols , 1982 .

[5]  Duncan Graham,et al.  The first SERRS multiplexing from labelled oligonucleotides in a microfluidics lab-on-a-chip. , 2004, Chemical communications.

[6]  L. Fruk,et al.  Detection of DNA probes using Diels Alder cycloaddition and SERRS. , 2003, The Analyst.

[7]  Tuan Vo-Dinh,et al.  Surface-enhanced Raman scattering detection of the breast cancer susceptibility gene BRCA1 using a silver-coated microarray platform , 2002 .

[8]  C. Mirkin,et al.  Nanoparticles with Raman spectroscopic fingerprints for DNA and RNA detection. , 2002, Science.

[9]  Igor Nabiev,et al.  Does Adsorption on the Surface of a Silver Colloid Perturb Drug/DNA Interactions? Comparative SERS, FT-SERS, and Resonance Raman Study of Mitoxantrone and Its Derivatives , 1995 .

[10]  J. Creighton,et al.  Plasma resonance — enhanced raman scattering by absorbates on gold colloids: The effects of aggregation , 1982 .

[11]  M. Klein,et al.  Raman scattering from carbon monoxide adsorbed on evaporated silver films , 1979 .

[12]  Duncan Graham,et al.  Simple multiplex genotyping by surface-enhanced resonance Raman scattering. , 2002, Analytical chemistry.

[13]  Synthesis of a benzotriazole phosphoramidite for attachment of oligonucleotides to metal surfaces , 2001 .

[14]  L. Fruk,et al.  A new approach to oligonucleotide labelling using Diels-Alder cycloadditions and detection by SERRS. , 2002, Chemical communications.

[15]  M. Albrecht,et al.  Anomalously intense Raman spectra of pyridine at a silver electrode , 1977 .

[16]  V. Shalaev,et al.  Direct and roughness-induced indirect transitions in photoemission from silver films , 1993 .

[17]  D A Weitz,et al.  Excitation spectra of surface-enhanced Raman scattering on silver-island films. , 1982, Optics letters.

[18]  Chad A. Mirkin,et al.  One-Pot Colorimetric Differentiation of Polynucleotides with Single Base Imperfections Using Gold Nanoparticle Probes , 1998 .

[19]  P. White,et al.  Characterization of the Surface of a Citrate-Reduced Colloid Optimized for Use as a Substrate for Surface-Enhanced Resonance Raman Scattering , 1995 .

[20]  Duncan Graham,et al.  Evaluation of surface-enhanced resonance Raman scattering for quantitative DNA analysis. , 2004, Analytical chemistry.

[21]  M. Natan,et al.  Nanoscale characterization of gold colloid monolayers:  a comparison of four techniques. , 1997, Analytical chemistry.

[22]  S. Sánchez‐Cortés,et al.  Adsorption of polyethyleneimine on silver nanoparticles and its interaction with a plasmid DNA: a surface-enhanced Raman scattering study. , 2002, Biomacromolecules.

[23]  H. Seki.  Surface enhanced Raman scattering of pyridine on different silver surfaces , 1982 .

[24]  Sanjay Tyagi,et al.  Molecular Beacons: Probes that Fluoresce upon Hybridization , 1996, Nature Biotechnology.

[25]  Y. Ozaki,et al.  Quantitative analysis of double-stranded DNA amplified by a polymerase chain reaction employing surface-enhanced Raman spectroscopy. , 1998, Applied optics.

[26]  I. Nabiev,et al.  Raman and surface-enhanced Raman scattering spectroscopy of bis-netropsins and their DNA complexes. , 2000, Biopolymers.

[27]  M. Albrecht,et al.  Plasma resonance enhancement of Raman scattering by pyridine adsorbed on silver or gold sol particles of size comparable to the excitation wavelength , 1979 .

[28]  Yang Wang,et al.  Near-Infrared Surface-Enhanced Raman Scattering (NIR SERS) on Colloidal Silver and Gold , 1994 .

[29]  K. Kneipp,et al.  Surface enhanced raman spectroscopy on nucleic acids and related compounds adsorbed on colloidal silver particles , 1991 .

[30]  C. A. Murray,et al.  Ultrahigh-Vacuum Studies of Enhanced Raman Scattering from Pyridine on Ag Surfaces , 1980 .

[31]  Duncan Graham,et al.  Surface‐Enhanced Resonance Raman Scattering as a Novel Method of DNA Discrimination , 2000 .

[32]  D. L. Jeanmaire,et al.  Surface raman spectroelectrochemistry: Part I. Heterocyclic, aromatic, and aliphatic amines adsorbed on the anodized silver electrode , 1977 .

[33]  P. Hildebrandt,et al.  Surface-enhanced resonance Raman spectroscopy of Rhodamine 6G adsorbed on colloidal silver , 1984 .

[34]  M. Procházka,et al.  Metallation kinetics of a free base porphyrin in surface-enhanced resonance Raman scattering active Ag colloid system as a probe of porphyrin–nucleic acids interaction , 1999 .

[35]  Duncan Graham,et al.  Selective detection of deoxyribonucleic acid at ultralow concentrations by SERRS , 1997 .

[36]  C. Nguyen,et al.  Molecular interactions of DNA-topoisomerase I and II inhibitor with DNA and topoisomerases and in ternary complexes: binding modes and biological effects for intoplicine derivatives. , 1994, Biochemistry.

[37]  G. A. Gachko,et al.  The study of the doxorubicin adsorbed onto chemically modified silver films by surface-enhanced spectroscopy , 2001 .

[38]  A. Wokaun,et al.  SERRS study of Acridine Orange and Its Binding to DNA Strands , 1994 .

[39]  M. Manfait,et al.  Selective interactions of ethidiums with G-quadruplex DNA revealed by surface-enhanced Raman scattering. , 2003, Analytical chemistry.

[40]  W. Smith,et al.  Synthesis of a benzotriazole azo dye phosphoramidite for labelling of oligonucleotides , 2003 .

[41]  R. P. Duyne,et al.  Surface enhanced raman and resonance raman spectroscopy in a non-aqueous electrochemical environment: Tris(2,2′-bipyridine)ruthenium(II) adsorbed on silver from acetonitrile , 1983 .

[42]  W. Smith,et al.  Detection and identification of labeled DNA by surface enhanced resonance Raman scattering. , 2000, Biopolymers.

[43]  Catherine J. Murphy,et al.  Oligonucleotide Adsorption to Gold Nanoparticles: A Surface-Enhanced Raman Spectroscopy Study of Intrinsically Bent DNA , 2001 .

[44]  R. G. Freeman,et al.  Preparation and Characterization of Au Colloid Monolayers , 1995 .

[45]  M. Fleischmann,et al.  Raman spectra of pyridine adsorbed at a silver electrode , 1974 .

[46]  M. Manfait,et al.  Comparative FT SERS, resonance Raman and SERRS studies of doxorubicin and its complex with DNA , 1995 .

[47]  Duncan Graham,et al.  SERRS as a more sensitive technique for the detection of labelled oligonucleotides compared to fluorescence. , 2004, The Analyst.

[48]  J. Chaires,et al.  Interaction of doxorubicin and its derivatives with DNA: Elucidation by resonance Raman and surface-enhanced resonance Raman spectroscopy , 1997 .