Needle-based reflection refractometry of scattering samples using coherence-gated detection.

We present a novel method for in situ refractive index measurement of scattering samples using a needle device. The device employs a fiber-based reflectance refractometer and coherence-gated detection of the reflected optical signal that eliminates scattering-dependent backreflection contributions. Additionally, birefringence changes induced by fiber movement are neutralized by randomizing the source polarizations and averaging the measured Fresnel reflection coefficients over many incident polarization states. Experimental measurements of Intralipid scattering solutions are presented and compared with Monte Carlo simulations.

[1]  R. Barer,et al.  Refractometry of Living Cells Part I. Basic Principles , 1954 .

[2]  S. P. Humphreys-Owen,et al.  Comparison of Reflection Methods for Measuring Optical Constants without Polarimetric Analysis, and Proposal for New Methods based on the Brewster Angle , 1961 .

[3]  G. M. Hale,et al.  Optical Constants of Water in the 200-nm to 200-microm Wavelength Region. , 1973, Applied optics.

[4]  G. Liese,et al.  Fiber-optic stylet for needle tip localization. , 1985, Applied optics.

[5]  D. F. Gray,et al.  Simultaneous thickness and group index measurement using optical low-coherence reflectometry , 1992, IEEE Photonics Technology Letters.

[6]  J. Fujimoto,et al.  Determination of the refractive index of highly scattering human tissue by optical coherence tomography. , 1995, Optics letters.

[7]  G. Meeten,et al.  Refractive index measurement of absorbing and turbid fluids by reflection near the critical angle , 1995 .

[8]  Andrew K. Dunn,et al.  Three-dimensional computation of light scattering from cells , 1996 .

[9]  J Beuthan,et al.  The spatial variation of the refractive index in biological cells. , 1996, Physics in medicine and biology.

[10]  Masato Ohmi,et al.  In vitro simultaneous measurement of refractive index and thickness of biological tissue by the low coherence interferometry , 2000, IEEE Transactions on Biomedical Engineering.

[11]  J. Fujimoto,et al.  Imaging needle for optical coherence tomography. , 2000, Optics letters.

[12]  Mark J Schnitzer,et al.  Gradient-index fiber-optic microprobes for minimally invasive in vivo low-coherence interferometry. , 2002, Optics letters.

[13]  Chunping Zhang,et al.  Simultaneous refractive index and thickness measurements of bio tissue by optical coherence tomography. , 2002, Journal of biomedical optics.

[14]  Shyam Singh Refractive Index Measurement and its Applications , 2002 .

[15]  Daniel L Marks,et al.  Projected index computed tomography. , 2003, Optics letters.

[16]  David D Sampson,et al.  Bifocal optical coherenc refractometry of turbid media. , 2003, Optics Letters.

[17]  Hamid Dehghani,et al.  The effects of internal refractive index variation in near-infrared optical tomography: a finite element modelling approach. , 2003, Physics in medicine and biology.

[18]  Jean-Michel Tualle,et al.  Derivation of the radiative transfer equation for scattering media with a spatially varying refractive index , 2003 .

[19]  S. Arridge,et al.  Validity conditions for the radiative transfer equation. , 2003, Journal of the Optical Society of America. A, Optics, image science, and vision.

[20]  J. C. D. T. Iniesta The radiative transfer equation , 2003 .

[21]  R Holland,et al.  Diagnostic accuracy for different strategies of image-guided breast intervention in cases of nonpalpable breast lesions , 2004, British Journal of Cancer.

[22]  David D. Sampson,et al.  Refractive index tomography of turbid media by bifocal optical coherence refractometry , 2004, SPIE BiOS.

[23]  B. Bouma,et al.  A portable, low coherence interferometry based instrument for fine needle aspiration biopsy guidance , 2005 .

[24]  Nirmala Ramanujam,et al.  Use of a multiseparation fiber optic probe for the optical diagnosis of breast cancer. , 2005, Journal of biomedical optics.

[25]  Jun Q. Lu,et al.  Determination of refractive indices of porcine skin tissues and intralipid at eight wavelengths between 325 and 1557 nm. , 2005, Journal of the Optical Society of America. A, Optics, image science, and vision.

[26]  Hanli Liu,et al.  Determination of reduced scattering coefficient of biological tissue from a needle-like probe. , 2005, Optics express.

[27]  Hamid Dehghani,et al.  Effects of refractive index on near-infrared tomography of the breast. , 2005, Applied optics.

[28]  Stephen A Boppart,et al.  Refractive index of carcinogen-induced rat mammary tumours , 2006, Physics in medicine and biology.

[29]  S. Boppart,et al.  Computational methods for analysis of human breast tumor tissue in optical coherence tomography images. , 2006, Journal of biomedical optics.

[30]  Freddy T. Nguyen,et al.  Needle-based refractive index measurement using low-coherence interferometry. , 2007, Optics letters.