Human immunodeficiency virus testing: Update

Human immunodeficiency virus (HIV) types 1 and 2 have been associated with the acquired immunodeficiency syndrome (AIDS). The detection of HIV infections is based on the screening of serum samples for the presence of antibodies to HIV proteins. Serum samples that test positive on screening must be assayed by a confirmatory test to provide a definitive report on the presence of HIV infection. This article reviews the currently available screening and confirmatory testing procedures and their limitations.