TWO FORMS OF ADENOSINE DEAMINASE IN PIG EPIDERMIS

Molecular heterogeneity of adenosine deaminase in pig epidermis is reported. Gel filtration of pig skin extracts with Sephadex G‐150 revealed the existence of two forms of adenosine deaminase. Molecular weights of the enzymes were around 30,000–40,000 and 300,000–350,000 respectively. Several catalytic properties of these two adenosine deaminases were quite similar: Km value, substrate specificity, pH activity profile, and the effect of coformycin, a strong inhibitor of adenosine deaminase.

[1]  H. Iizuka,et al.  THE EFFECTS OF db‐cAMP AND RELATED COMPOUNDS ON THE OUTGROWING EPIDERMIS IN VITRO , 1981, The Journal of dermatology.

[2]  D. Forsdyke,et al.  Isotope-dilution analysis of the effects of deoxyguanosine and deoxyadenosine on the incorporation of thymidine and deoxycytidine by hydroxyurea-treated thymus cells. , 1980, The Biochemical journal.

[3]  D. Martin,et al.  Alterations in deoxyribonucleotide metabolism in cultured cells with ribonucleotide reductase activities refractory to feedback inhibition by 2'-deoxyadenosine triphosphate. , 1980, The Journal of biological chemistry.

[4]  R. Ogura,et al.  Purification and characterization of hypoxanthine/guanine phosphoribosyltransferase in bovine snout epidermis. , 1980, The Journal of investigative dermatology.

[5]  P. Daddona,et al.  Human adenosine deaminase and its binding protein in normal and adenosine deaminase-deficient fibroblast cell strains. , 1980, The Journal of biological chemistry.

[6]  Tetsunori Kimura,et al.  ADENOSINE DEAMINASE ACTIVITY IN PERIPHERAL LYMPHOCYTES OF PSORIASIS AND SÉZARY'S SYNDROME , 1980 .

[7]  J. Taylor,et al.  Inhibitors of epidermal cell DNA synthesis in surviving pig skin in vitro. , 1980, The Journal of investigative dermatology.

[8]  P. Daddona,et al.  Human adenosine deaminase. Stoichiometry of the adenosine deaminase-binding protein complex. , 1979, Biochimica et biophysica acta.

[9]  T. Miyagawa.  Heterogeneity and Some Properties of β-Galactosidase from Newborn and Rat Epidermis , 1979 .

[10]  R. Peterfreund,et al.  Rabbit adenosine deaminase conversion proteins. Purification and characterization. , 1979, Biochemistry.

[11]  R. Ogura,et al.  Distribution of enzymes of nucleic acid metabolism in cow snout epidermis , 1978, The British journal of dermatology.

[12]  Trotta Pp,et al.  Characterization of adenosine deaminase from normal colon and colon tumors. Evidence for tumor-specific variants. , 1978 .

[13]  R. Parks,et al.  Potent inhibition of muscle 5'-AMP deaminase by the nucleoside antibiotics coformycin and deoxycoformycin. , 1977, Biochemical pharmacology.

[14]  Kizaki Harutoshi,et al.  Xanthine oxidase and guanase activities in normal and psoriatic epidermis , 1977 .

[15]  I. Holańska-Nalepa.  Assessment of a comparison of colorimetric methods used for oxytocinase determination. , 1977, Clinica chimica acta; international journal of clinical chemistry.

[16]  H. Kizaki,et al.  Xanthine oxidase and guanase activities in normal and psoriatic epidermis. , 1977, Clinica chimica acta; international journal of clinical chemistry.

[17]  H. Iizuka,et al.  Adenosine and adenine nucleotides stimulation of skin (epidermal) adenylate cyclase. , 1976, Biochimica et biophysica acta.

[18]  M. Weyden,et al.  Human adenosine deaminase. Distribution and properties. , 1976, The Journal of biological chemistry.

[19]  N. Delapp,et al.  Importance of pyrimidine nucleotide salvage pathways for DNA synthesis in skin. , 1976, The Journal of investigative dermatology.

[20]  J. Smyth,et al.  Role of adenosine deaminase in lymphocyte proliferation. , 1976, Clinical and experimental immunology.

[21]  S. Cha,et al.  Tight-binding inhibitors-II. Non-steady state nature of inhibition of milk xanthine oxidase by allopurinol and alloxanthine and of human erythrocytic adenosine deaminase by coformycin. , 1975, Biochemical pharmacology.

[22]  S. Cha,et al.  Tight-binding inhibitors-I. Kinetic behavior. , 1975, Biochemical pharmacology.

[23]  B. Flaxman,et al.  In vitro analysis of the control of keratinocyte proliferation in human epidermis by physiologic and pharmacologic agents. , 1975, The Journal of investigative dermatology.

[24]  H. Nishihara,et al.  [Multiple forms of human adenosine deaminase (author's transl)]. , 1975, Rinsho byori. The Japanese journal of clinical pathology.

[25]  R. Hirschhorn,et al.  Conversion of human erythrocyte-adenosine deaminase activity to different tissue-specific isozymes. Evidence for a common catalytic unit. , 1975, The Journal of clinical investigation.

[26]  R. Hirschhorn,et al.  Alterations in isozymes of adenosine deaminase during stimulation of human peripheral blood lymphocytes. , 1974, Cellular immunology.

[27]  W. Osborne,et al.  Partial purification and properties of the common inherited forms of adenosine deaminase from human erythrocytes. , 1973, The Biochemical journal.

[28]  S. Ishikawa,et al.  Multiple forms of human adenosine deaminase. I. Purification and characterization of two molecular species. , 1972, Biochimica et biophysica acta.

[29]  J. Fisher,et al.  Comparative studies of mammalian adenosine deaminases--some distinctive properties in higher mammals. , 1969, Comparative biochemistry and physiology.

[30]  T. Brady,et al.  Preparative isolation of the isoenzymes of adenosine deaminase from bovine mucosa by ion-exchange chromatography. , 1969, Biochimica et biophysica acta.

[31]  J. Fisher,et al.  Two different hepatic adenosine deaminases in the chicken. , 1968, Biochimica et biophysica acta.

[32]  J. G. Cory,et al.  Multiple forms of calf serum adenosine deaminase. , 1967, Archives of biochemistry and biophysics.

[33]  J. Hall.  Adenosine deaminase activity in lymphoid cells during antibody production. , 1963, The Australian journal of experimental biology and medical science.

[34]  W. Block,et al.  Studies of the enzymes of purine metabolism in skin. II. Nucleoside deaminases of rat skin. , 1954, The Journal of investigative dermatology.

[35]  O. H. Lowry,et al.  Protein measurement with the Folin phenol reagent. , 1951, The Journal of biological chemistry.

[36]  Kalckar Hm,et al.  Differential spectrophotometry of purine compounds by means of specific enzymes; studies of the enzymes of purine metabolism. , 1947 .

[37]  E. Conway,et al.  The deaminases of adenosine and adenylic acid in blood and tissues. , 1939, The Biochemical journal.