Polyhydroxyalkanotes (PHAs) production by using canola oil as carbon source from bacteria isolated near paper pulp industry

Samples were taken from paper pulp and mixed organic wastes of an industry for isolation of PHA producing bacterial strains. Quantitative analysis for PHA for bacterial strains was carried out by direct addition of sodium dodecyl sulphate (SDS) method. High PHA production ability was found in six strains belonging to Pseudomonas, Klebsiella, Bacillus, Streptococcus, Staphylococcus and Escherichia genera. The PHA production optimization of these six stains was done at various (NH 4 ) 2 SO 4 concentrations (0.2 %, 0.4 %, and 0.6%), pH (5, 6, and 7) and temperatures (4 o C, 37 o C, 45 o C). Strain WC20 belonging to Pseudomonas sp. was found to be a potential PHA producer at 37 o C, at 0.2 % of (NH 4 ) 2 SO 4 , using glucose as carbon source (PHA % ~28.35) and Canola Oil (PHA % ~16.06). PCR amplification of the phaC gene was also performed.