Effect of thermal blanching and of high pressure treatments on sweet green and red bell pepper fruits (Capsicum annuum L.)

Abstract The effect of pressure treatments of 100 and 200 MPa (10 and 20 min) and of thermal blanching at 70 °C, 80 °C and 98 °C (1 and 2.5 min), on sweet green and red bell peppers was compared. Pressure treated peppers showed a lower reduction on soluble protein and ascorbic acid contents. Red peppers presented even an increased content of ascorbic acid (15–20%), compared to the untreated peppers. Peroxidase and pectin methylesterase (whose activity was only quantifiable in green peppers) showed a higher stability to pressure treatments, particularly the latter enzyme, while polyphenol oxidase was inactivated to the same final level by the thermal blanching and pressure treatments. Pressure treatments resulted in comparable (in green pepper) to higher (in red pepper) microbial loads compared to blanching. Pressure treated green and red peppers presented similar to better firmness before and after tunnel freezing at −30 °C, compared to thermally blanched peppers, particularly those blanched at 98 °C. The results indicated that pressure treatments of 100 and 200 MPa can be used to produce frozen peppers with similar to better nutritional (soluble protein and ascorbic acid) and texture (firmness) characteristics, comparable activity of polyphenol oxidase and higher activity of pectin methylesterase, while pressure treated peppers show a higher level of peroxidase activity. It would be interesting to use higher pressures in future studies, as an attempt to cause a higher reduction on microbial load and on enzymatic activity.

[1]  A. Hagerman,et al.  Continuous spectrophotometric assay for plant pectin methyl esterase , 1986 .

[2]  P. Luning,et al.  Combined instrumental and sensory evaluation of flavor of fresh bell peppers (Capsicum annuum) harvested at three maturation stages , 1994 .

[3]  J. Selman Vitamin retention during blanching of vegetables , 1994 .

[4]  A. Carr,et al.  Toward a new recommended dietary allowance for vitamin C based on antioxidant and health effects in humans. , 1999, The American journal of clinical nutrition.

[5]  K. Waldron,et al.  Effect of Cooking and Pre‐Cooking on Cell‐Wall Chemistry in Relation to Firmness of Carrot Tissues , 1997 .

[6]  C. Nguyen-the,et al.  The microbiology of minimally processed fresh fruits and vegetables. , 1994, Critical reviews in food science and nutrition.

[7]  D. Barrett,et al.  Quality indicators in blanched, frozen, stored vegetables , 1995 .

[8]  G. Quaglia,et al.  Effect of High Pressure Treatments on Peroxidase Activity, Ascorbic Acid Content and Texture in Green Peas , 1996 .

[9]  Hernández,et al.  High-Pressure and Temperature Effects on Enzyme Inactivation in Tomato Puree. , 1998, Journal of agricultural and food chemistry.

[10]  S. Schwartz,et al.  Polygalacturonase, pectinesterase, and lipoxygenase activities in high-pressure-processed diced tomatoes. , 2001, Journal of agricultural and food chemistry.

[11]  Aurelio López-Malo,et al.  Minimally processed fruits and vegetables : fundamental aspects and applications , 2000 .

[12]  O. H. Lowry,et al.  Protein measurement with the Folin phenol reagent. , 1951, The Journal of biological chemistry.

[13]  E. J. Lourenço,et al.  Soluble and bound peroxidases from papaya fruit , 1990 .

[14]  O. Lamikanra Microbiology of Fresh-cut Produce , 2002 .

[15]  Eric R. Ziegel,et al.  Statistics and Chemometrics for Analytical Chemistry , 2004, Technometrics.

[16]  Vural Gökmen,et al.  Study of lipoxygenase and peroxidase as indicator enzymes in green beans: change of enzyme activity, ascorbic acid and chlorophylls during frozen storage , 2005 .

[17]  L. E. Jeremiah,et al.  Freezing Effects on Food Quality , 1996 .

[18]  M. A. Rao,et al.  A Kinetic Study of the Loss of Vitamin C, Color, and Firmness During Thermal Processing of Canned Peas , 1981 .

[19]  B. Estrada,et al.  Fruit development in Capsicum annuum: changes in capsaicin, lignin, free phenolics, and peroxidase patterns. , 2000, Journal of agricultural and food chemistry.

[20]  M. Hendrickx,et al.  Process stability of Capsicum annuum pectin methylesterase in model systems, pepper puree and intact pepper tissue , 2005 .

[21]  A. Robertson Official Methods of Analysis of the Association of Official Agricultural Chemists (7th ed.) , 1951 .

[22]  Chantal Smout,et al.  Effects of high-pressure pretreatment and calcium soaking on the texture degradation kinetics of carrots during thermal processing , 2006 .

[23]  M. Hendrickx,et al.  Effects of High Pressure on Enzymes Related to Food Quality , 1998 .

[24]  D. Ledward,et al.  Effect of high-pressure treatment on the activity of some polyphenoloxidases , 1996 .

[25]  M. Añón,et al.  Characterization and changes in polyphenol oxidase from eggplant fruit (Solanum melongena L.) during storage at low temperature , 2004 .

[26]  H. Daood,et al.  Ion-Pair Chromatography and Photodiode-Array Detection of Vitamin C and Organic Acids , 1994 .

[27]  M. Álvarez,et al.  Kinetics of thermal softening of potato tissue (cv. Monalisa) by water heating , 2001 .

[28]  O. Lamikanra,et al.  Fresh-Cut Fruits and Vegetables : Science, Technology, and Market , 2002 .

[29]  J. Antonio Torres,et al.  Commercial opportunities and research challenges in the high pressure processing of foods , 2005 .

[30]  M. Añón,et al.  Peroxidase from Strawberry Fruit (Fragaria ananassa Duch.): Partial Purification and Determination of Some Properties , 1995 .

[31]  C. Tonello,et al.  Les effets des hautes pressions sur la polyphénol oxydase des fruits , 1994 .

[32]  Dietrich Knorr,et al.  Monitoring the impact of high-pressure processing on the biosynthesis of plant metabolites using plant cell cultures , 1998 .

[33]  Jim W Hall,et al.  Ascorbic acid, dehydroascorbic acid and diketogulonic acid in frozen green peppers , 1978 .

[34]  P. Butz,et al.  Antioxidative capacity, nutrient content and sensory quality of orange juice and an orange-lemon-carrot juice product after high pressure treatment and storage in different packaging , 2001 .

[35]  M. Hendrickx,et al.  Temperature Sensitivity and Pressure Resistance of Mushroom Polyphenoloxidase , 1997 .

[36]  M. Cano,et al.  High pressure and temperature effects on enzyme inactivation in strawberry and orange products , 1997 .

[37]  L. Camardella,et al.  Pectin methylesterase inhibitor. , 2004, Biochimica et biophysica acta.

[38]  L. Howard,et al.  Firmness and phytochemical losses in pasteurized yellow banana peppers (Capsicum annuum) as affected by calcium chloride and storage. , 1999, Journal of agricultural and food chemistry.

[39]  J. Bouvier,et al.  Effect of ultra-high hydrostatic pressure on hydrosoluble vitamins , 1999 .

[40]  M. M. Rebolloso-Fuentes,et al.  Nutrient composition and antioxidant activity of 10 pepper (Capsicum annuun) varieties , 2006 .

[41]  M. Wall,et al.  Relationship of phytophthora fruit rot to fruit maturation and cuticle thickness of new Mexican-type peppers , 1993 .

[42]  G. González-Aguilar,et al.  Ascorbic Acid Content in Relation to Ascorbic Acid Oxidase Activity and Polyamine Content in Tomato and Bell Pepper Fruits During Development, Maturation and Senescence , 2001 .

[43]  J. Jen,et al.  Pectolytic Enzymes in Sweet Bell Peppers (Capsicum annuum L.) , 1984 .

[44]  E. E. Burns,et al.  Provitamin A and Ascorbic Acid Content of Fresh Pepper Cultivars (Capsicum annuum) and Processed Jalapeños , 1994 .

[45]  C. Zammer Gun-puffed vegetable snacks: a new way to eat your veggies , 1995 .

[46]  Dietrich Knorr,et al.  Effects of high-hydrostatic-pressure processes on food safety and quality , 1993 .

[47]  L. Howard,et al.  Flavonoids and Antioxidant Activity of Fresh Pepper (Capsicum annuum) Cultivars , 1995 .

[48]  R. Tharanathan,et al.  Post-harvest biochemical changes associated with the softening phenomenon in Capsicum annuum fruits , 1996 .

[49]  R. W. Berg,et al.  Quality and storage-stability of high-pressure preserved green beans , 2002 .

[50]  C. V. Morr,et al.  Changes in Soluble and Bound Peroxidase—IAA Oxidase During Tomato Fruit Development , 1982 .