In Vitro assessment of the Anti-viral effect of Curcumin longa on Herpes Simplex Virus Type 1

Antiviral drug resistance has become more prevalent in recent years. Therefore, this research aimed to investigate the antiviral effect of the of hydroalcoholic extract of Curcumin longa against human herpes simplex virus type 1. In this study, Curcumin longa hydroalcoholic extract was provided by the clevenger system. Cytotoxicity of Curcumin longa was determined on the Vero cell line. The antiviral effect of extract was evaluated by plaque reduction assays. The mean number of herpes simplex plaques was lower in group that received Curcumin longa extract with concentration of 2 mg/mL compared to that of the control group. Moreover, In contrast, acyclovir prevented 94.4% of virus plaque formation at the concentration of 2500 µg/mL. So, the finding of the present study showed the significant inhibitory effect of the Curcumin longa hydroalcoholic extract on HSV-1 replication. Curcumin longa hydroalcoholic extract or preparation can be recommended as a pharmaceutical source for controlling HSV-1 related diseases. However, it is necessary to investigate in-vivo the potential side effect of Curcumin longa.

[1]  Zhi-Li Huang,et al.  Curcumin exerts antinociceptive effects in a mouse model of neuropathic pain: Descending monoamine system and opioid receptors are differentially involved , 2012, Neuropharmacology.

[2]  Qi Zhou,et al.  Effect of curcumin on LDL oxidation in vitro, and lipid peroxidation and antioxidant enzymes in cholesterol fed rabbits. , 2011, International journal for vitamin and nutrition research. Internationale Zeitschrift fur Vitamin- und Ernahrungsforschung. Journal international de vitaminologie et de nutrition.

[3]  K. Chopra,et al.  Effect of insulin and its combination with resveratrol or curcumin in attenuation of diabetic neuropathic pain: participation of nitric oxide and TNF‐alpha , 2007, Phytotherapy research : PTR.

[4]  M. Bahmanyar,et al.  THE EFFECT OF ANTIVIRAL ACTIVITY OF GREEN SEAWEED FROM THE PERSIAN GULF, CAULERPA SERTULARIOIDES ON HERPES SIMPLEX VIRUS TYPE 1 , 2006 .

[5]  N. Futrakul,et al.  Combined effects of curcumin and vitamin C to protect endothelial dysfunction in the iris tissue of STZ-induced diabetic rats. , 2006, Clinical hemorheology and microcirculation.

[6]  P. Poma,et al.  Antitumor effects of curcumin, alone or in combination with cisplatin or doxorubicin, on human hepatic cancer cells. Analysis of their possible relationship to changes in NF-kB activation levels and in IAP gene expression. , 2005, Cancer letters.

[7]  J. Hudson,et al.  Antiviral and antimicrobial activities of Colombian medicinal plants. , 2001, Journal of ethnopharmacology.

[8]  M. Serra,et al.  Prevalence of hepatitis C virus in patients with lichen planus of the oral cavity and chronic liver disease. , 2000, European journal of oral sciences.

[9]  F. Parra,et al.  Evaluation of the antiviral activity of an aqueous extract from Phyllanthus orbicularis. , 2000, Journal of ethnopharmacology.

[10]  M. Arsenakis,et al.  Antiviral properties of isoborneol, a potent inhibitor of herpes simplex virus type 1. , 1999, Antiviral research.

[11]  S. Pancheva,et al.  Antiherpes effect of Melissa officinalis L. extracts. , 1993, Acta microbiologica Bulgarica.

[12]  J. Kavaliotis.  Acyclovir therapy in neonates. , 1992, The Journal of pediatrics.

[13]  J. D. Keys.  Chinese Herbs: Their Botany, Chemistry, and Pharmacodynamics , 1989 .

[14]  C. Crumpacker,et al.  Acyclovir-resistant herpes simplex virus infections in patients with the acquired immunodeficiency syndrome. , 1989, The New England journal of medicine.

[15]  L. Davis,et al.  Antiviral properties of garlic: in vitro effects on influenza B, herpes simplex and coxsackie viruses. , 1985, Planta medica.

[16]  P. Klapper,et al.  ACYCLOVIR IN HERPES ENCEPHALITIS , 1982, The Lancet.

[17]  R. E. Leach.  Dictionary of Microbiology , 1957 .