Characterization of tccP2 carried by atypical enteropathogenic Escherichia coli.

Atypical enteropathogenic Escherichia coli (EPEC) comprise an important group of paediatric pathogens. Atypical EPEC have reservoirs in farm and domestic animals where they can be either commensal or pathogenic; serogroup O26 is dominant in humans and animals. Central to intestinal colonization by EPEC is the translocation of the type III secretion system effector Tir into enterocytes, which following phosphorylation (Tir-Yp) recruits Nck to activate the N-WASP actin signalling cascade. The authors have recently shown that typical EPEC strains, belonging to the EPEC-2 lineage, carry a tir gene encoding Tir-Yp and can also use the alternative TccP2 actin-signalling cascade. The aim of this study was to determine if tccP2 is found in atypical EPEC isolated from human and farm animals. tccP2 was found at a frequency of 41% in non-O26 EPEC isolates and in 82.3% of the O26 strains. TccP2 of human and animal strains show high level of sequence identity. It is shown that most strains carry a tir gene encoding Tir-Yp. In addition the authors identified two new variants of tir genes in EPEC O104:H12 and NT:H19 strains.

[1]  Tetsuya Hayashi,et al.  TccP2-mediated subversion of actin dynamics by EPEC 2 – a distinct evolutionary lineage of enteropathogenic Escherichia coli , 2007, Microbiology.

[2]  Tetsuya Hayashi,et al.  TccP2 of O157:H7 and Non-O157 Enterohemorrhagic Escherichia coli (EHEC): Challenging the Dogma of EHEC-Induced Actin Polymerization , 2006, Infection and Immunity.

[3]  E. Allen-Vercoe,et al.  Amino Acid Residues within Enterohemorrhagic Escherichia coli O157:H7 Tir Involved in Phosphorylation, α-Actinin Recruitment, and Nck-Independent Pedestal Formation , 2006, Infection and Immunity.

[4]  M. Carlier,et al.  Characterization of TccP‐mediated N‐WASP activation during enterohaemorrhagic Escherichia coli infection , 2006, Cellular microbiology.

[5]  J. Leong,et al.  Enterohaemorrhagic Escherichia coli Tir requires a C‐terminal 12‐residue peptide to initiate EspFU‐mediated actin assembly and harbours N‐terminal sequences that influence pedestal length , 2006, Cellular microbiology.

[6]  T. Whittam,et al.  Allelic subtyping of the intimin locus (eae) of pathogenic Escherichia coli by fluorescent RFLP. , 2006, FEMS microbiology letters.

[7]  A. Whale,et al.  A novel category of enteropathogenic Escherichia coli simultaneously utilizes the Nck and TccP pathways to induce actin remodelling , 2006, Cellular microbiology.

[8]  E. Caron,et al.  Subversion of actin dynamics by EPEC and EHEC. , 2006, Current opinion in microbiology.

[9]  A. Whale,et al.  Distribution of tccP in Clinical Enterohemorrhagic and Enteropathogenic Escherichia coli Isolates , 2005, Journal of Clinical Microbiology.

[10]  B. Finlay,et al.  Modulation of Host Cytoskeleton Function by the Enteropathogenic Escherichia coli and Citrobacter rodentium Effector Protein EspG , 2005, Infection and Immunity.

[11]  J. Leong,et al.  Nck‐independent actin assembly is mediated by two phosphorylated tyrosines within enteropathogenic Escherichia coli Tir , 2005, Molecular microbiology.

[12]  L. Beutin,et al.  Investigation of domestic animals and pets as a reservoir for intimin- (eae) gene positive Escherichia coli types. , 2005, Veterinary microbiology.

[13]  E. Allen-Vercoe,et al.  A carboxy-terminal domain of Tir from enterohemorrhagic Escherichia coli O157:H7 (EHEC O157:H7) required for efficient type III secretion. , 2005, FEMS microbiology letters.

[14]  G. Frankel,et al.  Enteropathogenic Escherichia coli: unravelling pathogenesis. , 2005, FEMS microbiology reviews.

[15]  É. Oswald,et al.  TccP is an enterohaemorrhagic Escherichia coli O157:H7 type III effector protein that couples Tir to the actin‐cytoskeleton † , 2004, Cellular microbiology.

[16]  M. Woodward,et al.  Characterisation of attaching-effacing Escherichia coli isolated from animals at slaughter in England and Wales. , 2004, Veterinary microbiology.

[17]  J. Leong,et al.  EspFU is a translocated EHEC effector that interacts with Tir and N-WASP and promotes Nck-independent actin assembly. , 2004, Developmental cell.

[18]  Sudhir Kumar,et al.  MEGA3: Integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment , 2004, Briefings Bioinform..

[19]  Ulrich Dobrindt,et al.  Genomic islands in pathogenic and environmental microorganisms , 2004, Nature Reviews Microbiology.

[20]  J. Rodrigues,et al.  Reduced Etiological Role for Enteropathogenic Escherichia coli in Cases of Diarrhea in Brazilian Infants , 2004, Journal of Clinical Microbiology.

[21]  L. Trabulsi,et al.  10.321/eid0805.Typical and Atypical Enteropathogenic Escherichia coli , 2002, Emerging infectious diseases.

[22]  J. Leong,et al.  A tyrosine‐phosphorylated 12‐amino‐acid sequence of enteropathogenic Escherichia coli Tir binds the host adaptor protein Nck and is required for Nck localization to actin pedestals , 2002, Molecular microbiology.

[23]  B. Kenny,et al.  Phosphoserine modification of the enteropathogenic Escherichia coli Tir molecule is required to trigger conformational changes in Tir and efficient pedestal elongation , 2001, Molecular microbiology.

[24]  T. Pawson,et al.  Enteropathogenic E. coli Tir binds Nck to initiate actin pedestal formation in host cells , 2001, Nature Cell Biology.

[25]  G. Dougan,et al.  Intimin and the host cell--is it bound to end in Tir(s)? , 2001, Trends in microbiology.

[26]  B. Finlay,et al.  Recruitment of Cytoskeletal and Signaling Proteins to Enteropathogenic and Enterohemorrhagic Escherichia coli Pedestals , 2001, Infection and Immunity.

[27]  V. Sperandio,et al.  The Per regulon of enteropathogenic Escherichia coli : identification of a regulatory cascade and a novel transcriptional activator, the locus of enterocyte effacement (LEE)‐encoded regulator (Ler) , 1999, Molecular microbiology.

[28]  I. Connerton,et al.  Binding of intimin from enteropathogenic Escherichia coli to Tir and to host cells , 1999, Molecular microbiology.

[29]  B. Kenny.  Phosphorylation of tyrosine 474 of the enteropathogenic Escherichia coli (EPEC) Tir receptor molecule is essential for actin nucleating activity and is preceded by additional host modifications , 1999, Molecular microbiology.

[30]  B. Finlay,et al.  Enteropathogenic E. coli (EPEC) Transfers Its Receptor for Intimate Adherence into Mammalian Cells , 1997, Cell.

[31]  T. McDaniel,et al.  A cloned pathogenicity island from enteropathogenic Escherichia coli confers the attaching and effacing phenotype on E. coli K‐12 , 1997, Molecular microbiology.

[32]  T. McDaniel,et al.  Enteropathogenic Escherichia coli contains a putative type III secretion system necessary for the export of proteins involved in attaching and effacing lesion formation. , 1995, Proceedings of the National Academy of Sciences of the United States of America.

[33]  T. McDaniel,et al.  A genetic locus of enterocyte effacement conserved among diverse enterobacterial pathogens. , 1995, Proceedings of the National Academy of Sciences of the United States of America.

[34]  J. Kaper,et al.  Enteropathogenic Escherichia coli , 1992, Infection and immunity.

[35]  G. Schoolnik,et al.  An inducible bundle-forming pilus of enteropathogenic Escherichia coli. , 1991, Science.

[36]  J. Yu,et al.  A genetic locus of enteropathogenic Escherichia coli necessary for the production of attaching and effacing lesions on tissue culture cells. , 1990, Proceedings of the National Academy of Sciences of the United States of America.

[37]  S. Knutton,et al.  Adhesion of enteropathogenic Escherichia coli to human intestinal enterocytes and cultured human intestinal mucosa , 1987, Infection and immunity.