Corticotropin-releasing hormone in the human hypothalamus. Free-floating immunostaining method.

We have clearly demonstrated corticotropin-releasing hormone (CRH) immunoreactive cell bodies and nerve fibers in the human hypothalamus by immunocytochemistry using free-floating sections instead of paraffin-embedded sections. Human hypothalami were obtained at autopsy, fixed and cryostat-sectioned at 40 microns. Free-floating sections were immunostained with antibody to CRH using the Vector ABC system. Most of CRH immunoreactive nerve fibers from the paraventricular nucleus pass under the fornix, while some CRH immunoreactive nerve fibers pass beyond the fornix and some through the fornix. Then the CRH immunoreactive nerve fibers run downward, medially to the supraoptic nucleus and toward the pituitary stalk. This method of immunocytochemistry is a very sensitive and suitable means for immunocytochemical studies of neuropeptides in the human brain.

[1]  H. Ohtani,et al.  Immunoelectron microscopic study of corticotropin-releasing factor in the human hypothalamus and pituitary gland. , 1987, Neuroendocrinology.

[2]  Kazuhiro Takahashi,et al.  Suppression by glucocorticoid of the immunoreactivity of corticotropin-releasing factor and vasopressin in the paraventricular nucleus of rat hypothalamus , 1987, Neuroscience Letters.

[3]  T. Suda,et al.  Immunocytochemical identification of CRF in the human hypothalamus. , 1984, The Tohoku journal of experimental medicine.

[4]  H. Vaudry,et al.  Immunocytochemical localization of corticotropin-releasing factor-like immunoreactivity in the human hypothalamus , 1983, Neuroscience Letters.

[5]  A. Schally,et al.  The paraventriculo-infundibular corticotropin releasing factor (CRF) pathway as revealed by immunocytochemistry in long-term hypophysectomized or adrenalectomized rats , 1983, Regulatory Peptides.

[6]  D. Coy,et al.  Corticotropin-releasing factor stimulates secretion of melanocyte-stimulating hormone from the rat pituitary. , 1982, Science.

[7]  F. Bloom,et al.  Corticotropin releasing factor (CRF): immunoreactive neurones and fibers in rat hypothalamus , 1982, Regulatory Peptides.

[8]  D. Fellmann,et al.  Corticoliberin in rat brain: Immunocytochemical identification and localization of a novel neuroglandular system , 1982, Neuroscience Letters.

[9]  C. Turkelson,et al.  In vivo and in vitro release of ACTH by synthetic CRF , 1981, Peptides.

[10]  W. Vale,et al.  Characterization of a 41-residue ovine hypothalamic peptide that stimulates secretion of corticotropin and beta-endorphin , 1981 .

[11]  J. Adams.  Heavy metal intensification of DAB-based HRP reaction product. , 1981, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[12]  L. Swanson,et al.  Organization of ovine corticotropin-releasing factor immunoreactive cells and fibers in the rat brain: an immunohistochemical study. , 1983, Neuroendocrinology.

[13]  M. Brownstein,et al.  In vivo corticotropin-releasing factor-induced secretion of adrenocorticotropin, beta-endorphin, and corticosterone. , 1982, Endocrinology.