Epitope mapping of cytochrome P450 2B4 by peptide scanning.

Overlapping hexapeptides covering the whole sequence of the cytochrome P450 2B4 have been synthesized on the solid supports and tested by ELISA using the polyclonal antiserum against cytochrome 2B4. 70 hexapeptide fragments have been found to interact specifically with the antiserum, i.e. to possess antigenic activity. The mapped linear epitopes occupy about 43% of the whole sequence of 2B4. They presumably form clusters in the regions of No. 60-150, 210-300, 390-430 and 465-486 amino acid residues. The use of cytochrome P450 DataBase has allowed to classify the revealed antigenic determinants into absolutely specific for 2B4, specific only for 2B4 and 2B5, characteristic for 2B subfamily and widely distributed in family 2.