Three-dimensional representation of biological objects as reconstructed from sequential image planes: Part II - the reconstructed object space and its on-line integration with the image stack

A program package is described that: (1) handles a stack of several thousand aligned sequential photographic 2-D images as stored in an image processing system, (2) builds a database from information extracted from objects present in the stack of 2-D images, (3) transfers the database to an advanced graphic terminal, (4) reconstructs a 3-D object space, and (5) allows on-line interaction between the image processing system and the graphic terminal. The cell content of a prism of motor cerebral cortex of the cat is reconstructed as an application sample.