Evaluation of arthroscopic articular cartilage biopsy for osteoarthritis of the knee.

PURPOSE: Arthroscopic evaluation of the articular surface by visualization and palpation has proved to be unreliable; therefore, we investigated arthroscopically procured biopsy specimens and conventional sections by using the same histopathomorphologic techniques and compared the results for possible differences. METHODS: Cartilage biopsy specimens of 1.7 mm in diameter and conventional cartilage sections (pieces of 8 x 2 mm) were removed from the lateral femoral condyle of 53 osteoarthritis patients undergoing total knee arthroplasty. Biopsies and conventional sections were evaluated histologically by using Mankin's grading system and immunohistochemically by assessing the immunoreactivity of the chondrocytes to MMP-1 and MMP-3. RESULTS: The comparison between the biopsies and conventional sections revealed that there were no differences between these forms of obtaining specimens. Moreover, the expression of MMP-1 and MMP-3 in biopsy specimens showed a strong correlation with that in conventional sections. CONCLUSIONS: We conclude that in small biopsy specimens taken (e.g., during arthoscopy), the histological grading of osteoarthritis severity and the evaluation of MMP expression yield results similar to those obtained in conventional sections.

[1]  M. Dougados,et al.  Inter-observer reliability of the arthroscopic quantification of chondropathy of the knee. , 1998, Osteoarthritis and cartilage.

[2]  A. Roessner,et al.  Development of osteoarthritis in the knee joints of Wistar rats after strenuous running exercise in a running wheel by intracranial self-stimulation. , 1998, Pathology, research and practice.

[3]  A. Roessner,et al.  Immunohistochemical analysis of several proteolytic enzymes as parameters of cartilage degradation. , 1998, Pathology, research and practice.

[4]  A. Cole,et al.  Osteoarthritic lesions: involvement of three different collagenases. , 1997, Arthritis and rheumatism.

[5]  K. Ostergaard,et al.  Histologic/histochemical grading system for osteoarthritic articular cartilage: reproducibility and validity. , 1997, Arthritis and rheumatism.

[6]  A. Freemont,et al.  Gene expression of matrix metalloproteinases 1, 3, and 9 by chondrocytes in osteoarthritic human knee articular cartilage is zone and grade specific , 1997, Annals of the rheumatic diseases.

[7]  J. Steinmeyer,et al.  Pharmacological influence of antirheumatic drugs on proteoglycans from interleukin-1 treated articular cartilage. , 1997, Biochemical pharmacology.

[8]  F. Lafeber,et al.  Degenerated human articular cartilage at autopsy represents preclinical osteoarthritic cartilage: comparison with clinically defined osteoarthritic cartilage. , 1997, The Journal of rheumatology.

[9]  X. Ayral.  Diagnostic and quantitative arthroscopy: quantitative arthroscopy. , 1996, Bailliere's clinical rheumatology.

[10]  M. Dougados,et al.  Arthroscopic evaluation of chondropathy in osteoarthritis of the knee. , 1996, The Journal of rheumatology.

[11]  W. Blackburn,et al.  Cartilage imaging in osteoarthritis. , 1996, Seminars in arthritis and rheumatism.

[12]  W. Blackburn,et al.  Arthroscopic evaluation of knee articular cartilage: a comparison with plain radiographs and magnetic resonance imaging. , 1994, The Journal of rheumatology.

[13]  R. Ike,et al.  Detection of intraarticular abnormalities in osteoarthritis of the knee. A pilot study comparing needle arthroscopy with standard arthroscopy. , 1993, Arthritis and rheumatism.

[14]  R. Ike.  The role of arthroscopy in the differential diagnosis of osteoarthritis of the knee. , 1993, Rheumatic diseases clinics of North America.

[15]  K. Iwata,et al.  Localization of matrix metalloproteinase 3 (stromelysin) in osteoarthritic cartilage and synovium. , 1992, Laboratory investigation; a journal of technical methods and pathology.

[16]  K. Brandt,et al.  Radiographic grading of the severity of knee osteoarthritis: relation of the Kellgren and Lawrence grade to a grade based on joint space narrowing, and correlation with arthroscopic evidence of articular cartilage degeneration. , 2010, Arthritis and rheumatism.

[17]  E M Braunstein,et al.  Relationship between arthroscopic evidence of cartilage damage and radiographic evidence of joint space narrowing in early osteoarthritis of the knee. , 1991, Arthritis and rheumatism.

[18]  H. Dorfman,et al.  Biochemical and metabolic abnormalities in articular cartilage from osteo-arthritic human hips. II. Correlation of morphology with biochemical and metabolic data. , 1971, The Journal of bone and joint surgery. American volume.

[19]  H J Mankin,et al.  Biochemical and metabolic abnormalities in articular cartilage from osteo-arthritic human hips. , 1970, The Journal of bone and joint surgery. American volume.