A Giant Magnetoimpedance-Based Microfluidic System for Multiplex Immunological Assay

Microfluidic chip and giant magnetoimpedance (GMI)-related technology has developed quickly over the past decades in the field of biological detection. In this work, we designed and fabricated a GMIbased microfluidic system for screening of multiplex gastric cancer biomarkers. The microfluidic chip and GMI sensor were prepared by micro-electromechanical systems (MEMS) technology. This system can analyse 8 gastric cancer protein biomarkers simultaneously in less than 25 mins and offer more stable detection signal than conventional enzymological or fluorescent methods. The microfluidic chip was then tested in 150 clinical specimens and compared with enzyme-linked immunosorbent assay (ELISA) method. The results indicated no significant difference and excellent agreement. In short, the prototype of GMI-based microfluidic system has been developed successfully and showed promising potentials for parallel screening of cancer biomarkers.

[1]  Qingyun Zhang,et al.  Predictive Significance of Serum Level of Vascular Endothelial Growth Factor in Gastric Cancer Patients , 2016, BioMed research international.

[2]  Xiao Zhi,et al.  A Novel Electrochemical Microfluidic Chip Combined with Multiple Biomarkers for Early Diagnosis of Gastric Cancer , 2015, Nanoscale Research Letters.

[3]  A. Yang,et al.  CA72-4 combined with CEA, CA125 and CAl9-9 improves the sensitivity for the early diagnosis of gastric cancer. , 2014, Clinica chimica acta; international journal of clinical chemistry.

[4]  G. Roshandel,et al.  Diagnostic values of serum levels of pepsinogens and gastrin-17 for screening gastritis and gastric cancer in a high risk area in northern Iran. , 2014, Asian Pacific journal of cancer prevention : APJCP.

[5]  Xiao Zhi,et al.  A novel HBV genotypes detecting system combined with microfluidic chip, loop-mediated isothermal amplification and GMR sensors. , 2014, Biosensors & bioelectronics.

[6]  Tao Zhang,et al.  Capillary-driven surface-enhanced Raman scattering (SERS)-based microfluidic chip for abrin detection , 2014, Nanoscale Research Letters.

[7]  Xiao Zhi,et al.  Quick genotyping detection of HBV by giant magnetoresistive biochip combined with PCR and line probe assay. , 2012, Lab on a chip.

[8]  Ding Li,et al.  A prototype of giant magnetoimpedance-based biosensing system for targeted detection of gastric cancer cells. , 2011, Biosensors & bioelectronics.

[9]  Kan Wang,et al.  The potential of magnetic nanocluster and dual-functional protein-based strategy for noninvasive detection of HBV surface antibodies. , 2011, The Analyst.

[10]  Ding Li,et al.  Giant magnetoimpedance-based microchannel system for quick and parallel genotyping of human papilloma virus type 16/18 , 2010 .

[11]  Max Wiki,et al.  Optimal hybridization efficiency upon immobilization of oligonucleotide double helices. , 2009, The journal of physical chemistry. B.

[12]  L. V. Panina,et al.  Magneto‐impedance effect in amorphous wires , 1994 .

[13]  B. Guleng,et al.  Advances in Molecular Biomarkers for Gastric Cancer. , 2015, Critical reviews in eukaryotic gene expression.

[14]  T. Shimosegawa,et al.  Low serum levels of pepsinogen and gastrin 17 are predictive of extensive gastric atrophy with high-risk of early gastric cancer. , 2011, The Tohoku journal of experimental medicine.