22 week assessment of bladder acellular matrix as a bladder augmentation material in a porcine model.

Previous studies on the reconstruction of porcine bladder using bladder acellular matrix allograft (BAMA) have indicated positive preliminary results with respect to graft shrinkage and cellular repopulation. The current study was conducted to investigate the feasibility of using BAMA in a similar model of bladder reconstruction out to longer time frames (22 weeks). At predetermined time points, the macroscopic, histological and mechanical properties of explanted native and BAMA tissues were evaluated and compared. Macroscopically, contracture of the BAMA was observed. The peripheral regions of the grafts experienced extensive cellular repopulation. Towards the centre however, all grafts were consistently devoid of organized smooth muscle bundles and a well-developed urothelium. An alteration in both the amount and organization of collagen was also observed within this region. Significant differences (p < 0.05) in the rupture strain and the elastic modulus of the BAMA compared to native bladder tissue appear to correlate with macroscopic graft contracture as well as the fibroproliferative tissue response of the matrix.

[1]  J. Vacanti,et al.  Implantation in vivo and retrieval of artificial structures consisting of rabbit and human urothelium and human bladder muscle. , 1993, The Journal of urology.

[2]  S. Hayward,et al.  Mesenchymal-epithelial interactions in the bladder , 2004, World Journal of Urology.

[3]  M. C. Gerald,et al.  Age-related changes in sensitivity of rat urinary bladder to atonomic agents , 1984, Mechanisms of Ageing and Development.

[4]  W. Mcdougal.  Metabolic complications of urinary intestinal diversion. , 1992, The Journal of urology.

[5]  Y. Azuma,et al.  Alendronate inhibits osteopontin expression enhanced by parathyroid hormone-related peptide (PTHrP) in the rat kidney , 1998, Urological Research.

[6]  S. Hayward,et al.  Plasticity of the urothelial phenotype: effects of gastro-intestinal mesenchyme/stroma and implications for urinary tract reconstruction. , 2000, Differentiation; research in biological diversity.

[7]  M. Sato.  [Mechanical properties of living tissues]. , 1986, Iyo denshi to seitai kogaku. Japanese journal of medical electronics and biological engineering.

[8]  S. Hayward,et al.  Role of mesenchymal-epithelial interactions in normal bladder development. , 1996, The Journal of urology.

[9]  J. Vacanti,et al.  Formation of urothelial structures in vivo from dissociated cells attached to biodegradable polymer scaffolds in vitro. , 1992, The Journal of urology.

[10]  S. Badylak,et al.  Detrusor regeneration in the rat using porcine small intestinal submucosal grafts: functional innervation and receptor expression. , 1996, The Journal of urology.

[11]  J. Duckett,et al.  Active and passive compliance of the fetal bovine bladder. , 1997, The Journal of urology.

[12]  T F Lue,et al.  IN VITRO FUNCTIONAL PROPERTIES OF THE RAT BLADDER REGENERATED BY THE BLADDER ACELLULAR MATRIX GRAFT , 1998, The Journal of Urology.

[13]  A. Atala.  Engineering tissues and organs. , 1999, Current opinion in urology.

[14]  T. H. van der Kwast,et al.  Expression of growth factors and receptors during specific phases in regenerating urothelium after acute injury in vivo. , 1994, The American journal of pathology.

[15]  H. Yeger,et al.  Acellular matrix allograft small caliber vascular prostheses. , 1990, ASAIO transactions.

[16]  J M Lee,et al.  Effect of alternative crosslinking methods on the low strain rate viscoelastic properties of bovine pericardial bioprosthetic material. , 1990, Journal of biomedical materials research.

[17]  T F Lue,et al.  Functional rat bladder regeneration through xenotransplantation of the bladder acellular matrix graft. , 1998, British journal of urology.

[18]  D. Courtman,et al.  High Strain Rate Testing and Structural Analysis of Pericardial Bioprosthetic Materials , 1994 .

[19]  R. Levin,et al.  Comparison of urinary bladder function in 6 and 24 month male and female rats. , 1992, The Journal of urology.

[20]  A. Atala.  Tissue engineering of artificial organs. , 2000, Journal of endourology.

[21]  J. Southgate,et al.  Urothelial Tissue Regulation , 1999 .

[22]  S. Hayward,et al.  Regeneration of bladder urothelium, smooth muscle, blood vessels and nerves into an acellular tissue matrix. , 1996, The Journal of urology.

[23]  Griffith Dp,et al.  The prosthetic bladder--perhaps the technology has arrived. , 1992 .

[24]  S. Hayward,et al.  Understanding bladder regeneration: smooth muscle ontogeny. , 1999, The Journal of urology.

[25]  A. Atala.  Future perspectives in reconstructive surgery using tissue engineering. , 1999, The Urologic clinics of North America.

[26]  P. Merguerian,et al.  Acellular bladder matrix allografts in the regeneration of functional bladders: evaluation of large‐segment (> 24 cm2) substitution in a porcine model , 2000, BJU international.

[27]  P. Merguerian.  Urothelium-lined enteric segments , 2000, World Journal of Urology.

[28]  D. Griffith,et al.  The use of alloplastic biomaterials in bladder substitution. , 1992, The Journal of urology.

[29]  P. Merguerian,et al.  Regeneration of functional bladder substitutes using large segment acellular matrix allografts in a porcine model. , 2000, The Journal of urology.

[30]  D McComb,et al.  Development of a pericardial acellular matrix biomaterial: biochemical and mechanical effects of cell extraction. , 1994, Journal of biomedical materials research.

[31]  J. M. Lee,et al.  Thickness measurement of soft tissue biomaterials: a comparison of five methods. , 1996, Journal of biomechanics.

[32]  A. Brading,et al.  Smooth muscle of the bladder in the normal and the diseased state: pathophysiology, diagnosis and treatment. , 1997, Pharmacology & therapeutics.

[33]  R C Harruff,et al.  Experimental assessment of small intestinal submucosa as a bladder wall substitute. , 1995, Urology.

[34]  D W Hukins,et al.  Collagen orientation and molecular spacing during creep and stress-relaxation in soft connective tissues. , 1998, The Journal of experimental biology.

[35]  A. Atala,et al.  Biomaterials for tissue engineering , 2000, World Journal of Urology.

[36]  Anthony Atala,et al.  De novo reconstitution of a functional mammalian urinary bladder by tissue engineering , 1999, Nature Biotechnology.

[37]  A. Atala,et al.  Reconstructive options in genitourinary rhabdomyosarcoma. , 1996, The Journal of urology.

[38]  A. Keller,et al.  Crimp morphology in the fibre-forming collagens. , 1991, Matrix.

[39]  S. Raz,et al.  Complications of bladder substitution and continent urinary diversion. , 1989, Urology.

[40]  H. Yeger,et al.  Acellular matrix: a biomaterials approach for coronary artery bypass and heart valve replacement. , 1995, The Annals of thoracic surgery.

[41]  A. Wein,et al.  Mechanisms of bladder smooth-muscle hypertrophy and decompensation: lessons from normal development and the response to outlet obstruction , 1998, World Journal of Urology.

[42]  V. Monnier,et al.  Collagen Aging In Vitro by Nonenzymatic Glycosylation and Browning , 1984, Diabetes.

[43]  S. Badylak,et al.  Characterization of small intestinal submucosa regenerated canine detrusor: assessment of reinnervation, in vitro compliance and contractility. , 1996, The Journal of urology.

[44]  R. Clayman,et al.  Bladder wall substitution with synthetic and non-intestinal organic materials. , 1998, The Journal of urology.