Ethanol production by repeated-batch simultaneous saccharification and fermentation (SSF) of alkali-treated rice straw using immobilized Saccharomyces cerevisiae cells.

Repeated-batch simultaneous saccharification and fermentation (SSF) of alkali-treated rice straw using immobilized yeast was developed to produce ethanol. Saccharomyces cerevisiae cells were immobilized by entrapping in photocrosslinkable resin beads, and we evaluated the possibility of its reuse and ethanol production ability. In batch SSF of 20% (w/w) rice straw, the ethanol yields based on the glucan content of the immobilized cells were slightly low (76.9% of the theoretical yield) compared to free cells (85.2% of the theoretical yield). In repeated-batch SSF of 20% (w/w) rice straw, stable ethanol production of approx. 38gL(-1) and an ethanol yield of 84.7% were obtained. The immobilizing carrier could be reused without disintegration or any negative effect on ethanol production ability.

[1]  J. Krisch,et al.  Ethanol and acetic acid tolerance in free and immobilized cells of Saccharomyces cerevisiae and Acetobacter aceti , 1997, Biotechnology Letters.

[2]  A. Margaritis,et al.  High ethanol productivities using small Ca-alginate beads of immobilized cells of Zymomonas mobilis , 1981, Biotechnology Letters.

[3]  Freddy R. Delvaux,et al.  Immobilized yeast cell systems for continuous fermentation applications , 2006, Biotechnology Letters.

[4]  K. Kida,et al.  Ethanol production by repeated-batch fermentation at high temperature in a molasses medium containing a high concentration of total sugar by a thermotolerant flocculating yeast with improved salt-tolerance , 1997 .

[5]  H. Rehm,et al.  Relationship between fermentation capability and fatty acid composition of free and immobilized Saccharomyces cerevisiae , 2004, Applied Microbiology and Biotechnology.

[6]  Gi-wook Choi,et al.  Repeated-batch fermentation using flocculent hybrid, Saccharomyces cerevisiae CHFY0321 for efficient production of bioethanol , 2009, Applied Microbiology and Biotechnology.

[7]  R. Amutha,et al.  Production of ethanol from liquefied cassava starch using co-immobilized cells of Zymomonas mobilis and Saccharomyces diastaticus. , 2001, Journal of bioscience and bioengineering.

[8]  P. Piper,et al.  Weak acid adaptation: the stress response that confers yeasts with resistance to organic acid food preservatives. , 2001, Microbiology.

[9]  S. Limtong,et al.  Alginate-loofa as carrier matrix for ethanol production. , 2007, Journal of bioscience and bioengineering.

[10]  J. Shima,et al.  A strategy to prevent the occurrence of Lactobacillus strains using lactate-tolerant yeast Candida glabrata in bioethanol production , 2008, Journal of Industrial Microbiology & Biotechnology.

[11]  Masakazu Ike,et al.  Efficient Recovery of Glucose and Fructose via Enzymatic Saccharification of Rice Straw with Soft Carbohydrates , 2009, Bioscience, biotechnology, and biochemistry.

[12]  B. Saha,et al.  Lime pretreatment, enzymatic saccharification and fermentation of rice hulls to ethanol , 2008 .

[13]  Nibedita Sarkar,et al.  Bioethanol production from agricultural wastes: An overview , 2012 .

[14]  D. Yang,et al.  Enhanced production of bioethanol and ultrastructural characteristics of reused Saccharomyces cerevisiae immobilized calcium alginate beads. , 2011, Bioresource technology.

[15]  G. Zeeman,et al.  Pretreatments to enhance the digestibility of lignocellulosic biomass. , 2009, Bioresource technology.

[16]  Leif J. Jönsson,et al.  Influence of lignocellulose-derived aromatic compounds on oxygen-limited growth and ethanolic fermentation by Saccharomyces cerevisiae , 2000, Applied biochemistry and biotechnology.

[17]  S. Limtong,et al.  Ethanol production by repeated batch and continuous fermentations of blackstrap molasses using immobilized yeast cells on thin-shell silk cocoons , 2011 .

[18]  Ken Tokuyasu,et al.  A novel lime pretreatment for subsequent bioethanol production from rice straw--calcium capturing by carbonation (CaCCO) process. , 2010, Bioresource technology.

[19]  F. Alfani,et al.  Comparison of SHF and SSF processes for the bioconversion of steam-exploded wheat straw , 2000, Journal of Industrial Microbiology and Biotechnology.

[20]  Shi-zhong Liang,et al.  Study of sugarcane pieces as yeast supports for ethanol production from sugarcane juice and molasses , 2008, Journal of Industrial Microbiology & Biotechnology.