IC/microfluidic hybrid system for magnetic manipulation of biological cells

This paper introduces an integrated circuit (IC)/microfluidic hybrid system for magnetic manipulation of biological cells. The hybrid system consists of an IC and a microfluidic system fabricated on top. Biological cells attached to magnetic beads are suspended inside the microfluidic system that maintains biocompatibility. The IC contains a microcoil array circuit that produces spatially-patterned microscopic magnetic fields. Programmable, rapid reconfiguration of the field pattern made possible by the IC allows an efficient simultaneous manipulation of multiple individual bead-bound cells with precise position control. Two prototypes, SiGe/microfluidic and CMOS/microfluidic hybrid systems, validate the proposed approach.

[1]  A. Hassibi,et al.  A programmable electrochemical biosensor array in 0.18 /spl mu/m standard CMOS , 2005, ISSCC. 2005 IEEE International Digest of Technical Papers. Solid-State Circuits Conference, 2005..

[2]  G. Whitesides,et al.  Soft lithography in biology and biochemistry. , 2001, Annual review of biomedical engineering.

[3]  S. Bhatia,et al.  Tissue Engineering at the Micro-Scale , 1999 .

[4]  B. Eversmann,et al.  A 128 × 128 CMOS bio-sensor array for extracellular recording of neural activity , 2003 .

[5]  Luke P. Lee,et al.  Microfluidic application-specific integrated device for monitoring direct cell-cell communication via gap junctions between individual cell pairs , 2005 .

[6]  Donhee Ham,et al.  An IC/microfluidic hybrid microsystem for 2D magnetic manipulation of individual biological cells , 2005, ISSCC. 2005 IEEE International Digest of Technical Papers. Solid-State Circuits Conference, 2005..

[7]  M. Belleville,et al.  Active CMOS biochips: an electro-addressed DNA probe , 1998, 1998 IEEE International Solid-State Circuits Conference. Digest of Technical Papers, ISSCC. First Edition (Cat. No.98CH36156).

[8]  Hakho Lee,et al.  Manipulation of biological cells using a microelectromagnet matrix , 2004 .

[9]  Peter Fromherz,et al.  Neuron-semiconductor chip with chemical synapse between identified neurons. , 2004, Physical review letters.

[10]  P. Fromherz.  Joining ionics and electronics: semiconductor chips with ion channels, nerve cells, and brain tissue , 2005, ISSCC. 2005 IEEE International Digest of Technical Papers. Solid-State Circuits Conference, 2005..

[11]  Urs O. Häfeli,et al.  Scientific and clinical applications of magnetic carriers , 1997 .

[12]  S. Bhatia,et al.  Tissue Engineering at the MicroScale , 2000 .

[13]  N. Manaresi,et al.  A CMOS chip for individual cell manipulation and detection , 2003, 2003 IEEE International Solid-State Circuits Conference, 2003. Digest of Technical Papers. ISSCC..