Design and research of bone repair scaffold based on two-way fluid-structure interaction

BACKGROUND AND OBJECTIVE Porous bone repair scaffolds are an important method of repairing bone defects. Fluid flow in the scaffold plays a vital role in tissue differentiation and permeability and fluid shear stress (FSS) are two important factors. The differentiation of bone tissue depends on the osteogenic differentiation of cells, FSS affects cell proliferation and differentiation, and permeability affects the transportation of nutrients and metabolic waste. Therefore, it is necessary to better understand and analyze the FSS on the cell surface and the permeability of the scaffold to obtain better osteogenic performance. METHODS In this study, computational fluid dynamics (CFD) was used to analyze fluid flow in the scaffold. Three structures and nine scaffold unit cell models were designed and the cell models were loaded onto the scaffold surface. Considering cell deformability, the two-way fluid-structure interaction (FSI) method was used to evaluate the FSS on the cell surface. RESULTS The simulation results showed that as the pore size of the scaffold increases, its permeability increases and the FSS decreases. The FSS received on the cell surface was much larger than scaffold surface. Moreover the FSS on the cell surface was distributed in steps. CONCLUSIONS The results showed the permeability of all models matches that of human bone tissue. Based on the cell surface FSS as the criterion, it was found that the spherical-560 scaffold exhibited the best osteogenic performance. This provided a strategy to design a better bone repair scaffold from biological aspects.

[1]  Filippo Berto,et al.  Three-dimensional finite element analyses of functionally graded femoral prostheses with different geometrical configurations , 2014 .

[2]  A. Olivares,et al.  Finite element study of scaffold architecture design and culture conditions for tissue engineering. , 2009, Biomaterials.

[3]  D. Kaplan,et al.  Porosity of 3D biomaterial scaffolds and osteogenesis. , 2005, Biomaterials.

[4]  C. Jungreuthmayer,et al.  Deformation simulation of cells seeded on a collagen-GAG scaffold in a flow perfusion bioreactor using a sequential 3D CFD-elastostatics model. , 2009, Medical engineering & physics.

[5]  T. M. Keaveny,et al.  Dependence of Intertrabecular Permeability on Flow Direction and Anatomic Site , 1999, Annals of Biomedical Engineering.

[6]  H Van Oosterwyck,et al.  The effect of pore geometry on the in vitro biological behavior of human periosteum-derived cells seeded on selective laser-melted Ti6Al4V bone scaffolds. , 2012, Acta biomaterialia.

[7]  Stefaan W Verbruggen,et al.  Fluid flow in the osteocyte mechanical environment: a fluid–structure interaction approach , 2013, Biomechanics and Modeling in Mechanobiology.

[8]  M J Grimm,et al.  Measurements of permeability in human calcaneal trabecular bone. , 1997, Journal of biomechanics.

[9]  Hongwei Lu,et al.  Functionalized cell-free scaffolds for bone defect repair inspired by self-healing of bone fractures: A review and new perspectives. , 2019, Materials Science and Engineering C: Materials for Biological Applications.

[10]  Gerry L. Koons,et al.  Materials design for bone-tissue engineering , 2020, Nature Reviews Materials.

[11]  P. Pothacharoen,et al.  The 3D-Printed Bilayer’s Bioactive-Biomaterials Scaffold for Full-Thickness Articular Cartilage Defects Treatment , 2020, Materials.

[12]  H Van Oosterwyck,et al.  Prediction of permeability of regular scaffolds for skeletal tissue engineering: a combined computational and experimental study. , 2012, Acta biomaterialia.

[13]  Zhongmin Jin,et al.  Hydromechanical mechanism behind the effect of pore size of porous titanium scaffolds on osteoblast response and bone ingrowth , 2019 .

[14]  Cato T Laurencin,et al.  Tissue engineered bone: measurement of nutrient transport in three-dimensional matrices. , 2003, Journal of biomedical materials research. Part A.

[15]  A. Cortajarena,et al.  Mechanical performance of gelatin fiber mesh scaffolds reinforced with nano-hydroxyapatite under bone damage mechanisms , 2019, Materials Today Communications.

[16]  Fumihiko Kajiya,et al.  The alteration of a mechanical property of bone cells during the process of changing from osteoblasts to osteocytes. , 2008, Bone.

[17]  E. Maire,et al.  Fracture behavior of robocast HA/β-TCP scaffolds studied by X-ray tomography and finite element modeling , 2017 .

[18]  Jingdi Chen,et al.  New perspectives: In-situ tissue engineering for bone repair scaffold , 2020, Composites Part B: Engineering.

[19]  Davar Ali,et al.  Computational Fluid Dynamics Study of the Effects of Surface Roughness on Permeability and Fluid Flow-Induced Wall Shear Stress in Scaffolds , 2018, Annals of Biomedical Engineering.

[20]  Yan Gao,et al.  Simulation study of the effects of interstitial fluid pressure and blood flow velocity on transvascular transport of nanoparticles in tumor microenvironment , 2020, Comput. Methods Programs Biomed..

[21]  Xiaofeng Jia,et al.  Three-dimensional (3D) printed scaffold and material selection for bone repair. , 2019, Acta biomaterialia.

[22]  T. Vaughan,et al.  Quantification of fluid shear stress in bone tissue engineering scaffolds with spherical and cubical pore architectures , 2016, Biomechanics and modeling in mechanobiology.

[23]  M Browne,et al.  A prediction of cell differentiation and proliferation within a collagen-glycosaminoglycan scaffold subjected to mechanical strain and perfusive fluid flow. , 2010, Journal of biomechanics.

[24]  M G Haugh,et al.  A fluid–structure interaction model to characterize bone cell stimulation in parallel-plate flow chamber systems , 2013, Journal of The Royal Society Interface.

[25]  Qiang Zhang,et al.  The select of internal architecture for porous Ti alloy scaffold: A compromise between mechanical properties and permeability , 2020 .

[26]  Ming-Chuan Leu,et al.  Porous and strong bioactive glass (13–93) scaffolds fabricated by freeze extrusion technique , 2011 .

[27]  Seeram Ramakrishna,et al.  Development of nanocomposites for bone grafting , 2005 .

[28]  P R Fernandes,et al.  Permeability analysis of scaffolds for bone tissue engineering. , 2012, Journal of biomechanics.

[29]  Min-Ah Koo,et al.  Enhancement of human mesenchymal stem cell infiltration into the electrospun poly(lactic-co-glycolic acid) scaffold by fluid shear stress. , 2015, Biochemical and biophysical research communications.

[30]  Junda Zheng,et al.  Numerical simulation of particle transport and deposition in the pulmonary vasculature. , 2014, Journal of biomechanical engineering.

[31]  Umberto Morbiducci,et al.  A Survey of Methods for the Evaluation of Tissue Engineering Scaffold Permeability , 2013, Annals of Biomedical Engineering.

[32]  D. Lacroix,et al.  A dynamical study of the mechanical stimuli and tissue differentiation within a CaP scaffold based on micro-CT finite element models , 2011, Biomechanics and modeling in mechanobiology.

[33]  Yu-peng Lu,et al.  Interleukin-4 assisted calcium-strontium-zinc-phosphate coating induces controllable macrophage polarization and promotes osseointegration on titanium implant , 2020, Materials Science and Engineering: C.

[34]  Davar Ali,et al.  Finite element analysis of the effect of boron nitride nanotubes in beta tricalcium phosphate and hydroxyapatite elastic modulus using the RVE model , 2016 .

[35]  Josep A Planell,et al.  Computational modelling of the mechanical environment of osteogenesis within a polylactic acid-calcium phosphate glass scaffold. , 2009, Biomaterials.

[36]  Davar Ali,et al.  Finite element analysis of mechanical behavior, permeability and fluid induced wall shear stress of high porosity scaffolds with gyroid and lattice-based architectures. , 2017, Journal of the mechanical behavior of biomedical materials.

[37]  T. Vaughan,et al.  Multiscale fluid–structure interaction modelling to determine the mechanical stimulation of bone cells in a tissue engineered scaffold , 2015, Biomechanics and modeling in mechanobiology.

[38]  Damien Lacroix,et al.  In Vitro Bone Cell Models: Impact of Fluid Shear Stress on Bone Formation , 2016, Front. Bioeng. Biotechnol..

[39]  W. Ching,et al.  Mechanical properties, electronic structure and bonding of alpha- and beta-tricalcium phosphates with surface characterization. , 2010, Acta biomaterialia.

[40]  S. Seal,et al.  Carbon nanotube toughened hydroxyapatite by spark plasma sintering: Microstructural evolution and multiscale tribological properties , 2010 .

[41]  J. Fisher,et al.  Bone tissue engineering bioreactors: dynamic culture and the influence of shear stress. , 2011, Bone.

[42]  Antreas Kantaros,et al.  3D printing-assisted design of scaffold structures , 2016 .

[43]  I. Cengiz,et al.  Micro-CT based finite element modelling and experimental characterization of the compressive mechanical properties of 3-D zirconia scaffolds for bone tissue engineering. , 2019, Journal of the mechanical behavior of biomedical materials.

[44]  Y. Hao,et al.  Architectural design of Ti6Al4V scaffold controls the osteogenic volume and application area of the scaffold , 2020 .

[45]  Wenmiao Shu,et al.  3D bioactive composite scaffolds for bone tissue engineering , 2017, Bioactive materials.

[46]  J. H. Koolstra,et al.  3D-printed poly(Ɛ-caprolactone) scaffold with gradient mechanical properties according to force distribution in the mandible for mandibular bone tissue engineering. , 2020, Journal of the mechanical behavior of biomedical materials.