Development of interspecies cloned embryos in yak and dog.

Interspecies nuclear transfer (NT) could be an alternative to replicate animals when supply of recipient oocytes is limited or in vitro embryo production systems are incomplete. In the present study, embryonic development was assessed following interspecies NT of donor cumulus cells derived from yak and dog into the recipient ooplasm of domestic cow. The percentages of fusion and subsequent embryo development to the eight-cell stage of interspecies NT embryos were comparable to those of intraspecies NT embryos (cow-cow NT embryos). The percentage of development to blastocysts was significantly lower (p < 0.05) in yak-cow NT embryos than that in cow-cow NT embryos (10.9% vs. 39.8%). In dog-cow NT embryos, only one embryo (0.4%) developed to the blastocyst stage. These results indicate that interspecies NT embryos possess equally developmental competence to the eight-cell stage as intraspecies NT embryos, but the development to blastocysts is very low when dog somatic cells are used as the donor nuclei.

[1]  A. R. Caetano,et al.  Genome activation and developmental block in bovine embryos. , 2004, Animal reproduction science.

[2]  T. Otoi,et al.  Blastocysts derived from in vitro-fertilized cat oocytes after vitrification and dilution with sucrose. , 2004, Cryobiology.

[3]  R. Godke,et al.  Use of in vivo-recovered oocytes and adult somatic cells from the same donor for nuclear transfer in cattle , 2003, Veterinary Record.

[4]  J. Jenkins,et al.  Nuclear Transfer of Synchronized African Wild Cat Somatic Cells into Enucleated Domestic Cat Oocytes1 , 2003, Biology of reproduction.

[5]  Qing-Yuan Sun,et al.  Comparison of developmental capacity for intra‐ and interspecies cloned cat (Felis catus) embryos , 2003, Molecular reproduction and development.

[6]  Cai-Xia Yang,et al.  In vitro development and mitochondrial fate of macaca–rabbit cloned embryos , 2003, Molecular reproduction and development.

[7]  W. Hwang,et al.  Blastocyst development after intergeneric nuclear transfer of mountain bongo antelope somatic cells into bovine oocytes. , 2003, Cloning and stem cells.

[8]  A. Schnieke,et al.  Efficient Production of Transgenic Cloned Calves Using Preimplantation Screening , 2002, Biology of reproduction.

[9]  B. Barboni,et al.  Genetic rescue of an endangered mammal by cross-species nuclear transfer using post-mortem somatic cells , 2001, Nature Biotechnology.

[10]  R. Burghardt,et al.  Potential for cloning dogs. , 2001, Journal of reproduction and fertility. Supplement.

[11]  W. Farstad.  Assisted reproductive technology in canid species. , 2000, Theriogenology.

[12]  M. West,et al.  Cloning of an endangered species (Bos gaurus) using interspecies nuclear transfer. , 2000, Cloning.

[13]  T. Nagai,et al.  Production of calves by transfer of nuclei from cultured somatic cells obtained from Japanese black bulls. , 1999, Theriogenology.

[14]  E. Memili,et al.  Bovine oocyte cytoplasm supports development of embryos produced by nuclear transfer of somatic cell nuclei from various mammalian species. , 1999, Biology of reproduction.

[15]  C. Sumantri,et al.  Development of a simple, portable carbon dioxide incubator for in vitro production of bovine embryos. , 1999, Animal reproduction science.

[16]  S. Mitalipov,et al.  Establishment of pregnancy after the transfer of nuclear transfer embryos produced from the fusion of argali (Ovis ammon) nuclei into domestic sheep (Ovis aries) enucleated oocytes. , 1999, Cloning.

[17]  G. Schultz,et al.  Transition from maternal to embryonic control in early mammalian development: A comparison of several species , 1990, Molecular reproduction and development.