论文信息 - UV-Spectrophotometric Assay Method Development and Validation of Metronidazole in Bulk and Tablet Formulation

UV-Spectrophotometric Assay Method Development and Validation of Metronidazole in Bulk and Tablet Formulation

An easy, precise and accurate spectroscopic technique for the estimation of Metronidazole in pure form and tablet dosage form has been developed. The proposed method involves dissolving Metronidazole in 1(M) HCl solution and subjecting the resulting solution to UV Spectroscopic assessment. Absorption maximum was found to lie at about 277nm. Beer's law was obeyed in the concentration range of 2-10 2 µg/ml. Calibration curve showed linearity between absorbance and concentration as per line equation y=0.0347x-0.0071 with an R value of 0.9974. Validation was performed as ICH guidelines for linearity, accuracy and precision. The assay results were found to be in good agreement with label claim.

M. Dhua

[1] S. Ashour,et al. Simultaneous Determination of Miconazole Nitrate and Metronidazole in Different Pharmaceutical Dosage Forms by Gas Chromatography and Flame Ionization Detector (GC-FID) , 2010, International journal of biomedical science : IJBS.

[2] Lian Wang,et al. [Determination of common antibiotics and metronidazole in cosmetics by ultra performance liquid chromatography-tandem mass spectrometry]. , 2009, Se pu = Chinese journal of chromatography.

[3] C. Kaye,et al. A rapid and specific semi-micro method involving high-pressure liquid chromatography for the assay of metronidazole in plasma, saliva, serum, urine and whole blood. , 1980, British journal of clinical pharmacology.