Macrophage‐Dependent Response of Immune Human T Lymphocytes to PPD In Vitro Influence of HLA‐D Histocompatibility

The proliferative response of immune human T lymphocytes to low concentrations of purified protein derivative (PPD) in vitro was found to require the presence of macrophages. By coculturing different combinations of immune T lymphocytes and allogeneic macrophages with PPD in low concentration, optimal stimulation (that is, as efficient as the autologous combinations) was shown to require HLA‐D identity between the macrophages and responding T cells. Identity for HLA‐A and ‐B appeared to be of less or no importance. It is concluded that membrane structures encoded by HLA‐D or closely linked loci are involved in the human macrophage/T‐lymphocyte interaction in the proliferative response to PPD, much in the same way as the Ia antigens of rodents.

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