Human hepatocellular carcinoma is associated with quantitative and qualitative changes in glycolipids.

Changes of glycolipids in the naturally occurring human hepatocellular carcinoma tissues were studied. Neutral glycolipids (two fractions) and gangliosides from human hepatocellular carcinoma tissues and their adjacent uninvolved liver tissues from ten patients were purified and analyzed by high-performance thin-layer chromatography. Human hepatocellular carcinoma was shown to be associated with the extensive changes of the thin-layer chromatography patterns of both neutral glycolipids and gangliosides. Changes in neutral glycolipids were: (a) increased dihexaosylceramide with the concomitant decrease of trihexosylceramide and globoside, (b) increased ratio of the lower band of globoside to the upper band, and (c) increased expression of at least two "upper-phase" neutral glycolipids. Changes in gangliosides were: (a) increased expression of gangliosides migrating slower than GM3 on thin-layer chromatography with relative decrease of GM3, and (b) expression of tumor-associated new gangliosides, i.e., "neogangliosides".

[1]  T. Arima,et al.  Solid-phase radiolabeling of glycolipids with galactose oxidase and sodium boro[3H]hydride. , 1985, Analytical biochemistry.

[2]  S. Hakomori,et al.  Glycosphingolipids in detergent-insoluble substrate attachment matrix (DISAM) prepared from substrate attachment material (SAM). Their possible role in regulating cell adhesion. , 1984, Experimental Cell Research.

[3]  K. Isselbacher,et al.  Serum alpha-fetoprotein levels in human disease: perspective from a highly specific monoclonal radioimmunoassay. , 1984, Proceedings of the National Academy of Sciences of the United States of America.

[4]  J. Kirkwood,et al.  Gangliosides of normal and neoplastic human melanocytes. , 1984, Biochemical and biophysical research communications.

[5]  S. Hakomori,et al.  Novel fucolipids accumulating in human adenocarcinoma. I. Glycolipids with di- or trifucosylated type 2 chain. , 1984, The Journal of biological chemistry.

[6]  K. Sekiguchi,et al.  A monoclonal antibody directed to N-acetylneuraminosyl-alpha 2 leads to 6-galactosyl residue in gangliosides and glycoproteins. , 1983, The Journal of biological chemistry.

[7]  S. Hakomori,et al.  Glycosphingolipids as tumor-associated and differentiation markers. , 1983, Journal of the National Cancer Institute.

[8]  S. Hakomori,et al.  Human cancer-associated gangliosides defined by a monoclonal antibody (IB9) directed to sialosylα2→6galactosyl residue: A preliminary note , 1983 .

[9]  M. Lipinski,et al.  A glycolipid antigen associated with Burkitt lymphoma defined by a monoclonal antibody. , 1983, Science.

[10]  C. C. Blackburn,et al.  Carbohydrate-specific cell adhesion is mediated by immobilized glycolipids. , 1983, The Journal of biological chemistry.

[11]  B. Goff,et al.  Gangliosides of human acute leukemia cells. , 1983, Biochimica et biophysica acta.

[12]  M. Brockhaus,et al.  A monoclonal antibody-defined antigen associated with gastrointestinal cancer is a ganglioside containing sialylated lacto-N-fucopentaose II. , 1982, The Journal of biological chemistry.

[13]  S. Hakomori,et al.  Recent studies of glycolipid and glycoprotein profiles and characterization of the major glycolipid antigen in gastric cancer of a patient of blood group genotype pp (Tja-) first studied in 1951. , 1982, Cancer research.

[14]  S. Hakomori,et al.  The common structure in fucosyllactosaminolipids accumulating in human adenocarcinomas, and its possible absence in normal tissue. , 1982, Biochemical and biophysical research communications.

[15]  S. Hakomori,et al.  Characterization of a human melanoma-associated ganglioside antigen defined by a monoclonal antibody, 4.2. , 1982, The Journal of biological chemistry.

[16]  S. Hakomori,et al.  GM3 ganglioside induces hamster fibroblast growth inhibition in chemically-defined medium: ganglioside may regulate growth factor receptor function. , 1982, Biochemical and biophysical research communications.

[17]  S. Hakomori,et al.  Possible role of ceramide in defining structure and function of membrane glycolipids. , 1982, Proceedings of the National Academy of Sciences of the United States of America.

[18]  L. Old,et al.  GD3, a prominent ganglioside of human melanoma. Detection and characterisation by mouse monoclonal antibody , 1982, The Journal of experimental medicine.

[19]  J. David,et al.  Isolation of a guinea pig macrophage glycolipid with the properties of the putative migration inhibitory factor receptor. , 1982, The Journal of biological chemistry.

[20]  K. Uemura,et al.  The presence of blood group A-active glycolipids in cancer tissues from blood group O patients. , 1981, Biochimica et biophysica acta.

[21]  K. Uemura,et al.  Glycolipids of gastric cancer , 1981 .

[22]  S. Hakomori,et al.  Blood group A-like glycolipid and a novel Forssman antigen in the hepatocarcinoma of a blood group O individual. , 1981, Cancer research.

[23]  T. Yoneda,et al.  In vivo effect of anti-asialo GM1 antibody on natural killer activity , 1981, Nature.

[24]  W. J. Esselman,et al.  Modulation of the immune response by antigen-reactive lymphocytes after cultivation with gangliosides. , 1975, Journal of immunology.

[25]  C. Milstein,et al.  Continuous cultures of fused cells secreting antibody of predefined specificity , 1975, Nature.

[26]  T. Saito,et al.  Quantitative isolation of total glycosphingolipids from animal cells. , 1971, Journal of lipid research.

[27]  R. Brady,et al.  Gangliosides in DNA virus-transformed and spontaneously transformed tumorigenic mouse cell lines. , 1969, Proceedings of the National Academy of Sciences of the United States of America.

[28]  J. Aub,et al.  REACTIONS OF NORMAL AND TUMOR CELL SURFACES TO ENZYMES. I. WHEAT-GERM LIPASE AND ASSOCIATED MUCOPOLYSACCHARIDES. , 1963, Proceedings of the National Academy of Sciences of the United States of America.

[29]  J. Folch,et al.  A simple method for the isolation and purification of total lipides from animal tissues. , 1957, The Journal of biological chemistry.

[30]  J. Minna,et al.  Many monoclonal antibodies with an apparent specificity for certain lung cancers are directed against a sugar sequence found in lacto-N-fucopentaose III. , 1983, Archives of biochemistry and biophysics.

[31]  R. Yu,et al.  Gangliosides: structure, isolation, and analysis. , 1982, Methods in enzymology.

[32]  R. Laine,et al.  Isolation and Characterization of Membrane Glycosphingolipids , 1974 .

[33]  S. Hakomori,et al.  Glycolipids of hamster fibroblasts and derived malignant-transformed cell lines. , 1968, Proceedings of the National Academy of Sciences of the United States of America.

[34]  R. Spiro,et al.  [1] Analysis of sugars found in glycoproteins , 1966 .