Unusual Selective Response to Glycoprotein over Sugar Facilitates Ultrafast Universal Fluorescent Immunoassay of Biomarkers.

The enzyme-linked immunosorbent assay (ELISA) is widely used in clinical diagnostics. However, conventional ELISA is labor-intensive and lengthy. Herein, the sensitive detection of biomarkers with only one-step-incubation of 20 min is demonstrated, based on antibody-fused, boronic acid-decorated carbon nitride nanosheets. The decoration of carbon nitride nanosheets with boronic acid facilitates antibody binding at physiological conditions along with a concomitant fluorescence enhancement. The presence of target antigen results in decrement of the fluorescence, and ensures one-step immuno fluorescent detection. The immune recognition of antibody/target antigen in combination with glucose blocking ensure highly selective assay of the biomarkers. The protocol is validated by the assay of non-glycoprotein, glycoprotein and small molecular toxin targets. The multiplex target detection capability is demonstrated by the simultaneous assay of triple cardiac biomarker cTnI, Mb and CK-MB in human serum.

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