Regulation of normal and leukaemic lymphocyte transformation and recirculation by sodium periodate oxidation and sodium borohydride reduction.

MANY mitogenic agents1–5 when added to lymphocytes in vitro bind to saccharide-containing sites on cell membranes and cause blast transformation and subsequent incorporation of DNA precursors. Recently it has been observed by Novogrodsky and Katchalski6, using rat lymph node cells and by Parker et al.7, using human peripheral blood cells, that preincubation with sodium periodate (NaIO4), an oxidizing agent, induces blastogenesis comparable with that observed with phytohaemagglutinin (PHA).

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