Study on the biological effect of Tourmaline on the cell membrane of E. coli

The biological effect of tourmaline on the cell membrane of E. coli by microcalorimetry, fluorescence polarization, ion analysis and fourier transform infrared was studied. It was observed that tourmaline of low concentration can promote growth of the bacteria, while tourmaline of high concentration has inhibitory effects on E. coli. Fluorescence polarization has shown a significant decrease in membrane fluidity and the increase of permeability of cell membrane. The ion analysis result suggested that the absorbability of nutrition from the medium becomes easier. Thus, E. coli grew faster in the presence of tourmaline than the native. With high concentration of tourmaline, however, the growth of E. coli was inhibited because the selective barrier of cell membrane for the bacteria was seriously damaged. Besides, changes of the spectral profile of E. coli were observed, which has shown the damages of surface groups on the cell membrane, which is the molecular basis for the biological effect of tourmaline. Copyright © 2010 John Wiley & Sons, Ltd.

[1]  Qisui Wang,et al.  The damage of outer membrane of Escherichia coli in the presence of TiO2 combined with UV light. , 2010, Colloids and surfaces. B, Biointerfaces.

[2]  M. Raviv,et al.  Methodological aspects of microcalorimetry used to assess the dynamics of microbial activity during composting. , 2009, Bioresource technology.

[3]  M. Wiedenbeck,et al.  Boron isotope composition of tourmalinite and vein tourmalines associated with gold mineralization, Serra do Itaberaba Group, central Ribeira Belt, SE Brazil , 2009 .

[4]  K. Kobayashi,et al.  Chemical and boron isotopic variations of tourmaline in the Hnilec granite-related hydrothermal system, Slovakia: Constraints on magmatic and metamorphic fluid evolution , 2008 .

[5]  Misook Kang,et al.  Decomposition of 2-chlorophenol using a tourmaline–photocatalytic system , 2008 .

[6]  F. Ma,et al.  II4-P-005Investigation of the reduction performance of sulfate reducing bacteria enhanced by nano-meter/submicron tourmaline , 2008 .

[7]  L. Peng,et al.  Microcalorimetric studies of the action of Er3+ on Halobacterium halobium R1 growth , 2005, Biological Trace Element Research.

[8]  Oleg M. Sarkisov,et al.  Laser kinetic spectroscopy of the interfacial charge transfer between membrane cell walls of E. coli and TiO2 , 2006 .

[9]  V. Nadtochenko,et al.  Evidence for the mechanism of photocatalytic degradation of the bacterial wall membrane at the TiO2 interface by ATR-FTIR and laser kinetic spectroscopy. , 2005, Langmuir : the ACS journal of surfaces and colloids.

[10]  V. Nadtochenko,et al.  Dynamics of E. coli membrane cell peroxidation during TiO2 photocatalysis studied by ATR-FTIR spectroscopy and AFM microscopy , 2005 .

[11]  Zijian Guo,et al.  Novel polynuclear platinum adducts detected during the reactions of [Pt(Met-S,N)Cl2] with gamma-glutathione and L-cysteine. , 2004, Journal of inorganic biochemistry.

[12]  L. Peng,et al.  Study on biological effect of La3+ on Escherichia coli by atomic force microscopy , 2004 .

[13]  Ji Zhijiang,et al.  Observation of spontaneous polarization of tourmaline , 2003 .

[14]  K. Watson,et al.  Membrane fatty acid composition and membrane fluidity as parameters of stress tolerance in yeast. , 1997, Canadian journal of microbiology.

[15]  M. Lida,et al.  Tourmaline and lithium niobate reaction with water , 1994 .

[16]  W. Coleman,et al.  Cloning and characterization of the Escherichia coli K-12 rfa-2 (rfaC) gene, a gene required for lipopolysaccharide inner core synthesis , 1993, Journal of bacteriology.

[17]  M. Petersheim,et al.  An ionotropic phase transition in phosphatidylcholine: cation and anion cooperativity. , 1987, Biochimica et biophysica acta.

[18]  S. Yamaguchi.  Surface electric fields of tourmaline , 1983 .

[19]  H. Hauser,et al.  The interaction of ions with phosphatidylcholine bilayers , 1977 .