Drp1 is dispensable for apoptotic cytochrome c release in primed MCF10A and fibroblast cells but affects Bcl‐2 antagonist‐induced respiratory changes

Dynamin‐related protein 1 (Drp1) mediates mitochondrial fission and is thought to promote Bax/Bak‐induced cytochrome c release during apoptosis. Conformationally active Bax, Bak and Bax/Bak‐activating BH3‐only proteins, such as Bim, are restrained by anti‐apoptotic Bcl‐2 proteins in cells that are ‘primed for death’. Inhibition of Bcl‐2/Bcl‐xL/Bcl‐w by the antagonist ABT‐737 causes rapid apoptosis of primed cells. Hence, we determined whether Drp1 is required for cytochrome c release, respiratory alterations and apoptosis of cells that are already primed for death.

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