Fifty-eight investigators from the fields of biochemistry, physiology, cardiology, nuclear medicine, and physics met to discuss the development of metabolic imaging techniques for application to cardiovascular and pulmonary studies in health and disease. The workshop was sponsored by the Division of Heart and Vascular Diseases, National Heart, Lung, and Blood Institute and was held on September 16 to 18 in Bethesda, Maryland, in facilities provided by the American College of Cardiology. This report summarizes the presentations and discussions and presents recommendations for future studies.