Imaging characteristic optimization of digital holographic microscopy for onion epidermal cells

The optimization of image resolution for digital holographic scanning imaging of biological cells is investigated. Digital holographic scanning imaging experiments on the upper epidermal cells of onions are performed to demonstrate the validity of resolution optimization algorithm. In the experiments, the holograms of the upper epidermal cells of onion are recorded at a certain scanning rate, and then are processed by using the resolution optimization algorithm. As a result, the phase images of the onion epidermal cells with higher contrast and resolution are obtained. According to the synthetic holograms, the changes of cell nucleus and actin microfilament inside onion’s epidermal cells are displayed. In addition, the dehydration process and plasmolysis phenomenon inside onion epidermal cells are also exhibited by recording longterm scanning holograms of living epidermal cells. The experimental results demonstrate that image quality of living onion epidermal cells can be improved by optimizing the algorithms.

[1]  J. W. Goodman,et al.  Digital Image Formation From Electronically Detected Holograms , 1967, Other Conferences.

[2]  D. Keefer,et al.  Improved immunoglobulin-enzyme bridge method for light microscopic demonstration of hormone-containing cells of the rat adenohypophysis , 1975, Histochemistry.

[3]  F. Zernike.  Phase contrast, a new method for the microscopic observation of transparent objects , 1942 .

[4]  Steffi Ketelhut,et al.  Prospects and challenges of quantitative phase imaging in tumor cell biology , 2016, SPIE BiOS.

[5]  A. Ozcan,et al.  Lensfree on-chip microscopy over a wide field-of-view using pixel super-resolution , 2010, Optics express.

[6]  P. Ferraro,et al.  Spatio-temporal scanning modality for synthesizing interferograms and digital holograms. , 2014, Optics express.

[7]  M. Gross,et al.  Numerical heterodyne holography with two-dimensional photodetector arrays. , 2000, Optics letters.

[8]  T M Jovin,et al.  Fluorescence digital imaging microscopy in cell biology. , 1985, Science.

[9]  Manon Rostykus,et al.  Compact lensless subpixel resolution large field of view microscope. , 2018, Optics letters.

[10]  Samuel T. Thurman,et al.  Multiplexed synthetic-aperture digital holography , 2015 .

[11]  Xi-bin Yang,et al.  High-throughput imaging of zebrafish embryos using a linear-CCD-based flow imaging system. , 2017, Biomedical optics express.

[12]  J. H. Massig,et al.  Digital off-axis holography with a synthetic aperture. , 2002, Optics letters.