Efficacy of oral administration of a modified-live Salmonella Dublin vaccine in calves.

OBJECTIVE To determine the efficacy a modified-live Salmonella Dublin vaccine administered PO in an extralabel manner in the prevention of diseases associated with Salmonella Dublin infection. DESIGN Randomized clinical trial. ANIMALS 288 preweaned Holstein dairy calves on a commercial dairy farm. PROCEDURES Calves were orally administered either 2 mL of a commercially available, modified-live Salmonella Dublin vaccine (n = 140) or a placebo (148) at 3 and 10 days of age. Signs of diarrhea and depression were recorded daily. Weight gain between 3 days of age and time of weaning was measured. Fecal samples from clinically depressed or diarrheic calves and fresh tissues samples from calves that died were submitted for bacterial culture of Salmonella organisms. RESULTS Salmonella organisms were isolated from samples of 1.4% (2/140) and 3.4% (5/148) of calves receiving the vaccine and placebo, respectively. Additionally, 57.1% (80/140) and 60.1 % (89/148) of the vaccinated and control calves, respectively, had at least 1 day with an abnormal fecal score. Calves receiving the vaccine and placebo were not significantly different in terms of overall morbidity rate, Salmonella-specific morbidity rate, or average daily gain. Adverse reactions related to administration of the vaccine were not seen. The attenuated vaccine strain was not isolated from any fecal or tissue samples. CONCLUSIONS AND CLINICAL RELEVANCE This method of vaccination was safe in young Holstein calves, although it was not effective in reducing the incidence of disease or improving weight gain on this farm. However, the power of this study was limited by a low incidence of clinical salmonellosis.

[1]  J M Sargeant,et al.  Methodological quality and completeness of reporting in clinical trials conducted in livestock species. , 2009, Preventive veterinary medicine.

[2]  H. Erb,et al.  Variability in Holstein heifer heart-girth measurements and comparison of prediction equations for live weight. , 2007, Preventive veterinary medicine.

[3]  T. Besser,et al.  Antimicrobial resistance in Salmonella enterica serovar Dublin isolates from beef and dairy sources. , 2007, Veterinary microbiology.

[4]  L. Babiuk,et al.  Mucosal delivery of vaccines in domestic animals. , 2006, Veterinary research.

[5]  G. Entrican,et al.  Advances in mucosal vaccination , 2004, Animal Health Research Reviews.

[6]  Y T Gröhn,et al.  Salmonella Dublin infection in dairy cattle: risk factors for becoming a carrier. , 2004, Preventive veterinary medicine.

[7]  N Toft,et al.  Evaluation of an indirect serum ELISA and a bacteriological faecal culture test for diagnosis of Salmonella serotype Dublin in cattle using latent class models , 2004, Journal of applied microbiology.

[8]  E. Dueger,et al.  Evaluation of an autogenous Salmonella bacterin and a modified live Salmonella serotype Choleraesuis vaccine on a commercial dairy farm. , 2001, American journal of veterinary research.

[9]  R. Curtiss,et al.  Live oral avirulent Salmonella vaccines. , 1993, Veterinary microbiology.

[10]  T. Segall,et al.  Oral vaccination of calves with an aromatic-dependent Salmonella dublin (O9,12) hybrid expressing O4,12 protects against S. dublin (O9,12) but not against Salmonella typhimurium (O4,5,12) , 1993, Infection and immunity.

[11]  C. Wray,et al.  Transmission of salmonellae among calves penned individually , 1991, The Veterinary Record.

[12]  Bradford P. Smith.  Large Animal Internal Medicine , 1990 .

[13]  C. Hjerpe.  Bovine vaccines and herd vaccination programs. , 1990, The Veterinary clinics of North America. Food animal practice.

[14]  S. Hietala,et al.  Chemiluminescence of bovine alveolar macrophages as an indicator of developing immunity in calves vaccinated with aromatic-dependent Salmonella. , 1985, Veterinary microbiology.

[15]  B. Stocker,et al.  Aromatic-dependent Salmonella dublin as a parenteral modified live vaccine for calves. , 1984, American journal of veterinary research.

[16]  B. Stocker,et al.  Aromatic-dependent Salmonella typhimurium are non-virulent and effective as live vaccines , 1981, Nature.

[17]  Fu Ping.  Surveillance for Food-Borne Disease Outbreaks-United States:1998-2002 , 2009 .

[18]  J. House,et al.  CHAPTER 25 – Salmonellosis in Ruminants , 2009 .

[19]  R. Smith,et al.  Vaccinating calves: new information on the effects of maternal immunity. , 2007 .

[20]  L. Eberly,et al.  Herd-level factors associated with isolation of Salmonella in a multi-state study of conventional and organic dairy farms I. Salmonella shedding in cows. , 2005, Preventive veterinary medicine.

[21]  G. Dougan,et al.  Immune responses in calves immunised orally or subcutaneously with a live Salmonella typhimurium aro vaccine. , 1998, Vaccine.

[22]  J S Cullor,et al.  The effect of Escherichia coli J5 and modified live Salmonella dublin vaccines in artificially reared neonatal calves. , 1995, Vaccine.

[23]  B. Stocker,et al.  Vaccination of calves with orally administered aromatic-dependent Salmonella dublin. , 1993, American journal of veterinary research.

[24]  D. Love,et al.  Immunizing efficacy of aromatic-dependent Salmonella dublin in mice and calves. , 1991, Comparative immunology, microbiology and infectious diseases.

[25]  G. Dougan,et al.  Oral vaccination of calves against experimental salmonellosis using a double aro mutant of Salmonella typhimurium. , 1991, Vaccine.

[26]  C. Braden,et al.  Surveillance for foodborne-disease outbreaks--United States, 1998-2002. , 2006, Morbidity and mortality weekly report. Surveillance summaries.