Lumen formation during angiogenesis in vitro involves phagocytic activity, formation and secretion of vacuoles, cell death, and capillary tube remodelling by different populations of endothelial cells

We have utilised an in vitro model of angiogenesis to investigate the morphological changes which occur during the formation of a lumen in capillary tubes.

[1]  J. Flanagan,et al.  Identification and cloning of ELF-1, a developmentally expressed ligand for the Mek4 and Sek receptor tyrosine kinases , 1994, Cell.

[2]  L Orci,et al.  In vitro rapid organization of endothelial cells into capillary-like networks is promoted by collagen matrices , 1983, The Journal of cell biology.

[3]  W. Reilly,et al.  A variant vascular endothelial cell line with altered growth characteristics , 1979, Journal of cellular physiology.

[4]  D. Cheresh,et al.  Integrin α v β 3 antagonists promote tumor regression by inducing apoptosis of angiogenic blood vessels , 1994, Cell.

[5]  K. Ley,et al.  Differential adhesion of granulocytes to five distinct phenotypes of cultured microvascular endothelial cells. , 1992, Microvascular research.

[6]  N. Bruce,et al.  Structural and functional reserve of steroidogenic membranes in stimulated luteal cells of pregnant rats. , 1991, Journal of anatomy.

[7]  J. Gamble,et al.  Regulation of in vitro capillary tube formation by anti-integrin antibodies , 1993, The Journal of cell biology.

[8]  G. Striker,et al.  Factors influencing endothelial cell proliferation in vitro , 1978, Journal of cellular physiology.

[9]  D. Noden Embryonic origins and assembly of blood vessels. , 1989, The American review of respiratory disease.

[10]  J. Behrens,et al.  Cultured microvascular endothelial cells (MVEC) differ in cytoskeleton, expression of cadherins and fibronectin matrix. A study under the influence of interferon-gamma. , 1993, Journal of cell science.

[11]  S. Egginton,et al.  Angiogenesis in Skeletal Muscle , 1996 .

[12]  C. Little,et al.  Antibodies to β1‐integrins cause alterations of aortic vasculogenesis, in vivo , 1992 .

[13]  Judah Folkman,et al.  Angiogenesis in vitro , 1980, Nature.

[14]  E. Hay,et al.  Epithelia suspended in collagen gels can lose polarity and express characteristics of migrating mesenchymal cells , 1982, The Journal of cell biology.

[15]  S. Okisaka,et al.  Formation of capillary-like tubes by vascular endothelial cells cocultivated with keratocytes. , 1992, Investigative ophthalmology & visual science.

[16]  J. McGeachie,et al.  Angiogenesis in the developing corpus luteum of pregnant rats: A stereologic and autoradiographic study , 1988, The Anatomical record.

[17]  T. K. Hunt,et al.  Role of Platelets and Fibrin in the Healing Sequence: An In Vivo Study of Angiogenesis and Collagen Synthesis , 1982, Annals of surgery.

[18]  Jonathan Bard,et al.  COLLAGEN SUBSTRATA FOR STUDIES ON CELL BEHAVIOR , 1972, The Journal of cell biology.

[19]  H. Kleinman,et al.  Role of laminin and basement membrane in the morphological differentiation of human endothelial cells into capillary-like structures , 1988, The Journal of cell biology.

[20]  M. Iruela-Arispe,et al.  Differential expression of extracellular proteins is correlated with angiogenesis in vitro. , 1991, Laboratory investigation; a journal of technical methods and pathology.