Optimization of Bacterial Poly – β – Hydroxy Butyrate (PHB) Production from Different Industrial Waste using Central Composite Design

Poly (ß-hydroxybutyrate) (PHB) belongs to a family of microbial energy/carbon storage compounds collectively known as poly hydroxyalkanoates. About 150 different PHAs have been identified as constituents of bacterial polyesters. PHB is natural, biodegradable polyester which is accumulated in the form of intracellular granules by a variety of heterotrophic and autotrophic aerobic bacteria, photosynthetic anaerobic bacteria, gliding bacteria, Actinomycetes sp, cyanobacteria and recently an anaerobic fatty acid oxidizing gram negative bacterium. Until now there are only few reports on marine PHAs producing microorganisms. With this background an optimization of media components for the effective PHB production using different industrial waste has been attempted for this study.

[1]  Y. Chisti,et al.  Disruption of Alcaligenes latus for Recovery of Poly(β-hydroxybutyric acid): Comparison of High-Pressure Homogenization, Bead Milling, and Chemically Induced Lysis , 1998 .

[2]  E. Meighen,et al.  Biosynthesis of poly-3-hydroxybutyrate in the luminescent bacterium, Vibrio harveyi, and regulation by the lux autoinducer, N-(3-hydroxybutanoyl)homoserine lactone. , 1994, The Journal of biological chemistry.

[3]  H. Chang,et al.  A Simple Method for Recovery of Microbial $Poly-{\beta}-hydroxybutyrate$ by Alkaline Solution Treatment , 1995 .

[4]  Arnold L. Demain,et al.  Manual of Industrial Microbiology and Biotechnology , 1986 .

[5]  A. Arun,et al.  Utilization of various industrial wastes for the production of poly-β-hydroxy butyrate (PHB) by Alcaligenes eutrophus , 2006 .

[6]  C. Chavarie,et al.  Extraction of poly-3-hydroxybutyrate using chlorinated solvents , 1994 .

[7]  S. Lee Bacterial polyhydroxyalkanoates , 1996, Biotechnology and bioengineering.

[8]  Manjusri Misra,et al.  Recent advances in biodegradable nanocomposites. , 2005, Journal of nanoscience and nanotechnology.

[9]  I. Noda,et al.  Preparation and properties of a novel class of polyhydroxyalkanoate copolymers. , 2005, Biomacromolecules.

[10]  C. Chavarie,et al.  Recovery of poly-3-hydroxyalkanoic acid granules by a surfactant-hypochlorite treatment , 1990 .

[11]  T. Gerngross,et al.  How green are green plastics? , 2000, Scientific American.

[12]  R. Smith Biodegradable polymers for industrial applications , 2005 .

[13]  C. Beltrami Life is plastic. , 2008, Blood.

[14]  M. Zinn,et al.  Bacterial poly(hydroxyalkanoates) as a source of chiral hydroxyalkanoic acids. , 2005, Biomacromolecules.

[15]  Y. Doi,et al.  STAINING METHOD OF POLY(3-HYDROXYALKANOIC ACIDS) PRODUCING BACTERIA BY NILE BLUE , 2004 .

[16]  Y. Chisti Strategies in Downstream Processing , 2008 .

[17]  G. W. Small Spectrometric Identification of Organic Compounds , 1992 .

[18]  G. Subramanian,et al.  Bioseparation and Bioprocessing , 1998 .

[19]  R. Weiner Biopolymers from marine prokaryotes. , 1997, Trends in biotechnology.