Kinetic analysis of the effects of products removal on the hydrolysis of penicillin G by immobilised penicillin acylase

The effects of products removal on the hydrolysis of penicillin G by immobilised penicillin acylase were studied by kinetic simulation. Analysis of the substrate and products inhibition data from the literature for this kinetically controlled reaction shows that the removal of phenylacetic acid (PAA) as produced in this reaction has little effect on the reaction rate.

[1]  F. Le Goffic,et al.  The immobilization of penicillin G acylase on chitosan. , 1989, Biotechnology and bioengineering.

[2]  H. Sivaraman,et al.  PENICILLIN ACYLASE: ENZYME PRODUCTION AND ITS APPLICATION IN THE MANUFACTURE OF 6-APA , 1989 .

[3]  S. B. Lee,et al.  Reaction kinetics and mechanisms of penicillin amidase: A comparative study by computer simulation , 1982 .

[4]  R. Crichton,et al.  Stabilisation and immobilisation of penicillin amidase , 1989, FEBS letters.

[5]  Peter Dunnill,et al.  Conversion of benzylpenicillin to 6‐aminopenicillanic acid in a batch reactor and continuous feed stirred tank reactor using immobilized penicillin amidase , 1973 .

[6]  P Dunnill,et al.  The isolation and kinetics of penicillin amidase from Escherichia coli. , 1972, Biochimica et biophysica acta.

[7]  R. Quintero,et al.  Characterization and use of a penicillin acylase biocatalyst. , 2007, Journal of chemical technology and biotechnology.

[8]  K. Suga,et al.  Hydrolysis of penicillin G by combination of immobilized penicillin acylase and electrodialysis , 1992, Biotechnology and bioengineering.

[9]  C. Kutzbach,et al.  Preparation and General Properties of Crystalline Penicillin Acylase from Escherichia coli ATCC 11 105 , 2009, Hoppe-Seyler's Zeitschrift fur physiologische Chemie.

[10]  S. W. Carleysmith,et al.  Kinetic behavior of immobilized Penicillin acylase , 1980 .

[11]  T. Scheper,et al.  An integrated process for the production and biotransformation of penicillin. , 1992, Journal of biotechnology.