Identification of a Metagenome-Assembled Genome of an Uncultured Methyloceanibacter sp. Strain Acquired from an Activated Sludge System Used for Landfill Leachate Treatment

Using metagenome sequencing, a nearly complete genome sequence was retrieved for the uncultured Methyloceanibacter sp. strain A49, recovered from an activated sludge system used for landfill leachate treatment at a closed landfill site. The total size and encoded sequences are 3,407,434 bp and 3,280 genes, respectively. ABSTRACT Using metagenome sequencing, a nearly complete genome sequence was retrieved for the uncultured Methyloceanibacter sp. strain A49, recovered from an activated sludge system used for landfill leachate treatment at a closed landfill site. The total size and encoded sequences are 3,407,434 bp and 3,280 genes, respectively.

[1]  Hiroyuki Ogata,et al.  KofamKOALA: KEGG Ortholog assignment based on profile HMM and adaptive score threshold , 2019, bioRxiv.

[2]  Soo-Je Park,et al.  The characteristics and comparative analysis of methanotrophs reveal genomic insights into Methylomicrobium sp. enriched from marine sediments. , 2018, Systematic and applied microbiology.

[3]  James R. Cole,et al.  The Microbial Genomes Atlas (MiGA) webserver: taxonomic and gene diversity analysis of Archaea and Bacteria at the whole genome level , 2018, Nucleic Acids Res..

[4]  Yasukazu Nakamura,et al.  DFAST: a flexible prokaryotic genome annotation pipeline for faster genome publication , 2017, Bioinform..

[5]  P. de Vos,et al.  New Methyloceanibacter diversity from North Sea sediments includes methanotroph containing solely the soluble methane monooxygenase. , 2016, Environmental microbiology.

[6]  Connor T. Skennerton,et al.  CheckM: assessing the quality of microbial genomes recovered from isolates, single cells, and metagenomes , 2015, Genome research.

[7]  Kunihiko Sadakane,et al.  MEGAHIT: an ultra-fast single-node solution for large and complex metagenomics assembly via succinct de Bruijn graph , 2014, Bioinform..

[8]  Anders F. Andersson,et al.  Binning metagenomic contigs by coverage and composition , 2014, Nature Methods.

[9]  Björn Usadel,et al.  Trimmomatic: a flexible trimmer for Illumina sequence data , 2014, Bioinform..

[10]  M. Hattori,et al.  Methyloceanibacter caenitepidi gen. nov., sp. nov., a facultatively methylotrophic bacterium isolated from marine sediments near a hydrothermal vent. , 2014, International journal of systematic and evolutionary microbiology.

[11]  K. Kawai,et al.  A Catalytic Role of XoxF1 as La3+-Dependent Methanol Dehydrogenase in Methylobacterium extorquens Strain AM1 , 2012, PloS one.

[12]  Steven L Salzberg,et al.  Fast gapped-read alignment with Bowtie 2 , 2012, Nature Methods.

[13]  Robert D. Finn,et al.  HMMER web server: interactive sequence similarity searching , 2011, Nucleic Acids Res..

[14]  Gonçalo R. Abecasis,et al.  The Sequence Alignment/Map format and SAMtools , 2009, Bioinform..

[15]  Tom O. Delmont,et al.  Distributed under Creative Commons Cc-by 4.0 Anvi'o: an Advanced Analysis and Visualization Platform for 'omics Data , 2022 .