Antioxidant effect of Inonotus obliquus.

The mushroom Inonotus obliquus (Fr.) Pilát (Hymenochaetaceae), has been widely used as a folk medicine in Russia, Poland and most of the Baltic countries. The purpose of this study was to elucidate the antioxidant capacities of Inonotus obliquus. Four extracts from the fungus were evaluated for antioxidant activity against the 1,1-diphenyl-2-picrylhydrazyl (DPPH), superoxide, and peroxyl radicals. The polyphenolic extract had a strong antioxidant activity, and the extract containing triterpenoids and steroids presented a relatively strong antioxidant effect. The polysaccharide extract, however, was inactive. The protective effects of these four extracts were assessed against hydrogen peroxide-induced oxidative stress using a human keratinocyte cell line, HaCaT. Our results show that the polyphenolic extract protected these cells against hydrogen peroxide-induced oxidative stress, while the polysaccharide, triterpenoid and steroid extracts were ineffective. Additionally, the remnant polyphenolic and low molecular weight polysaccharide extracts showed a weakly protective effect at a concentration of 50 microg/ml. Our results indicate that Inonotus obliquus has the capacity to scavenge free radicals at concentrations higher than 5 microg/ml and that the polyphenolic extract can protect cells against oxidative stress.

[1]  V. Shcherba,et al.  [Melanin complex of the fungus Inonotus obliquus]. , 2000, Prikladnaia biokhimiia i mikrobiologiia.

[2]  M. Polansky,et al.  Isolation and characterization of polyphenol type-A polymers from cinnamon with insulin-like biological activity. , 2004, Journal of agricultural and food chemistry.

[3]  V. Ooi,et al.  Free radical scavenging activities of mushroom polysaccharide extracts. , 1997, Life sciences.

[4]  R. Hiltunen,et al.  Ergosterol peroxide, an active compound from Inonotus radiatus. , 1989, Planta medica.

[5]  B. Ames,et al.  Detection of picomole levels of hydroperoxides using a fluorescent dichlorofluorescein assay. , 1983, Analytical biochemistry.

[6]  V. Babitskaya,et al.  Melanin complex of the fungusInonotus obliquus , 2000, Applied Biochemistry and Microbiology.

[7]  T. Kuusi,et al.  Spectrophotometric assay for total peroxyl radical-trapping antioxidant potential in human serum. , 1997, Journal of lipid research.

[8]  S. W. Kim,et al.  Antioxidant Activity of Ergosterol Peroxide (5,8-Epidioxy-$5\alpha,8\alpha$-ergosta-6,22E-dien-3$\beta$-ol) in Armillariella mellea , 1999 .

[9]  R. Zheng,et al.  Scavenging effects of phenylpropanoid glycosides from Pedicularis on superoxide anion and hydroxyl radical by the spin trapping method(95)02255-4. , 1996, Biochemical pharmacology.

[10]  M. Mizuno,et al.  Fucogalactan isolated from Sarcodon aspratus elicits release of tumor necrosis factor-alpha and nitric oxide from murine macrophages. , 2000, Immunopharmacology.

[11]  E. Fernandes,et al.  Antioxidant activity of Hypericum androsaemum infusion: scavenging activity against superoxide radical, hydroxyl radical and hypochlorous acid. , 2002, Biological & pharmaceutical bulletin.

[12]  R. Schmidt,et al.  The antioxidant activity of Chinese herbs for eczema and of placebo herbs--I. , 1997, Journal of ethnopharmacology.

[13]  K. Kondo,et al.  Antioxidant ability of various flavonoids against DPPH radicals and LDL oxidation. , 2001, Journal of nutritional science and vitaminology.

[14]  J. Buttriss,et al.  A review of the epidemiological evidence for the ‘antioxidant hypothesis’ , 2004, Public Health Nutrition.

[15]  Sang Wook Kim,et al.  Antioxidant Activity of Ergosterol Peroxide (5,8-Epidioxy-5α,8α-ergosta-6,22E-dien-3β-ol) in Armillariella mellea , 1999 .

[16]  G. Rastelli,et al.  Activity of Polyphenolic Crude Extracts as Scavangers of Superoxide Radicals and Inhibitors of Xanthine Oxidase , 1992, Planta medica.

[17]  J. Mau,et al.  Antioxidant properties of several medicinal mushrooms. , 2002, Journal of agricultural and food chemistry.

[18]  J. Rzymowska.  The effect of aqueous extracts from Inonotus obliquus on the mitotic index and enzyme activities. , 1998, Bollettino chimico farmaceutico.

[19]  Xiaoming Feng,et al.  Three New Triterpenoids from Fuscoporia Obliqua , 2001, Journal of Asian natural products research.

[20]  P. Lemieux,et al.  Optimization of the tetrazolium-based colorimetric assay for the measurement of cell number and cytotoxicity. , 1988, International journal of immunopharmacology.

[21]  Lee Seng Teik,et al.  Anti-oxidant effects of the extracts from the leaves of Chromolaena odorata on human dermal fibroblasts and epidermal keratinocytes against hydrogen peroxide and hypoxanthine-xanthine oxidase induced damage. , 2001, Burns : journal of the International Society for Burn Injuries.

[22]  L. McCune,et al.  Antioxidant activity in medicinal plants associated with the symptoms of diabetes mellitus used by the indigenous peoples of the North American boreal forest. , 2002, Journal of ethnopharmacology.