Complex Formation of Poly-L-lysine with Poly(acrylic acid)

Conformation-directing interactions between poly-L-lysine (PLL) and poly(acrylic acid) (PAA) in aqueous media have been studied as a function of pH by circular dichroism spectroscopy. It was found that PAA reacts with PLL stoichio-metrically independent of the molecular weight of the PAA and induces α-helix formation for PLL. The induced circular dichroism spectra of the complex have been measured by using acridine orange as a chromophore. The results, together with the CD spectra in the ultraviolet region, suggest that PAA which is capable of forming a left-handed super helix binds to the core composed of a right-handed α-helix of PLL.

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