Development of an AC electrokinetics-based immunoassay system for on-site serodiagnosis of infectious diseases

Abstract This paper presents a lab-chip immunoassay system that is based on AC electrothermal effect. It uses a poly (dimethylsiloxane) based microfluidic cartridge as the disposable immunoreactor, on which key processes such as reagent delivery, incubation and washing are performed using low voltage AC signals. A low cost, reconfigurable detection module is constructed based on light emitting diode induced fluorescence. Along with the employed AC electrothermal effect, the developed diagnostic system has demonstrated a much shorter incubation time than conventional pressure driven flow system. A tenfold acceleration in detection is achieved while safely differentiating between the positive and negative primary antibodies with large margin. Other merits of this immunoassay system include portability, rapid detection and low reagent consumption.

[1]  Juan G Santiago,et al.  Detection of 100 aM fluorophores using a high-sensitivity on-chip CE system and transient isotachophoresis. , 2007, Analytical chemistry.

[2]  Kangning Ren,et al.  Miniaturized high throughput detection system for capillary array electrophoresis on chip with integrated light emitting diode array as addressed ring-shaped light source. , 2009, Lab on a chip.

[3]  Castellanos,et al.  Fluid flow induced by nonuniform ac electric fields in electrolytes on microelectrodes. II. A linear double-layer analysis , 2000, Physical review. E, Statistical physics, plasmas, fluids, and related interdisciplinary topics.

[4]  Jiehong Wu,et al.  A Simple Method to Integrate In Situ Nano-Particle Focusing With Cantilever Detection , 2007, IEEE Sensors Journal.

[5]  Chun Yang,et al.  DC-biased AC-electroosmotic and AC-electrothermal flow mixing in microchannels. , 2009, Lab on a chip.

[6]  Segyeong Joo,et al.  A portable microfluidic flow cytometer based on simultaneous detection of impedance and fluorescence. , 2010, Biosensors & bioelectronics.

[7]  H. B. Halsall,et al.  Microfluidic immunosensor systems. , 2005, Biosensors & bioelectronics.

[8]  Yi Zhang,et al.  An integrated fluorescence detection system for lab-on-a-chip applications. , 2007, Lab on a chip.

[9]  Meng Lian,et al.  Ultrafast micropumping by biased alternating current electrokinetics , 2009 .

[10]  M. Lian,et al.  AC electrothermal manipulation of conductive fluids and particles for lab-chip applications. , 2007, IET nanobiotechnology.

[11]  Jiehong Wu,et al.  Micropumping of biofluids by alternating current electrothermal effects , 2007 .

[12]  R. Chien,et al.  Sample stacking in laboratory-on-a-chip devices. , 2001, Journal of chromatography. A.

[13]  Jiehong Wu,et al.  Numerical study of in situ preconcentration for rapid and sensitive nanoparticle detection. , 2010, Biomicrofluidics.

[14]  F. Zenhausern,et al.  A compact LED-based module for DNA capillary electrophoresis , 2008 .

[15]  S. Jacobson,et al.  Integrated system for rapid PCR-based DNA analysis in microfluidic devices. , 2000, Analytical chemistry.

[16]  Q. Fang,et al.  A low-cost light-emitting diode induced fluorescence detector for capillary electrophoresis based on an orthogonal optical arrangement. , 2009, Talanta.

[17]  Guoqing Hu,et al.  Miniaturized immunoassay microfluidic system with electrokinetic control. , 2006, Biosensors & bioelectronics.

[18]  Marin Sigurdson,et al.  AC electrothermal enhancement of heterogeneous assays in microfluidics. , 2007, Lab on a chip.

[19]  J. Wu,et al.  Interactions of electrical fields with fluids: laboratory-on-a-chip applications. , 2008, IET nanobiotechnology.

[20]  Jie Wu,et al.  ac electro-osmotic micropump by asymmetric electrode polarization , 2008 .

[21]  Dazhi Wang,et al.  Electrothermal stirring for heterogeneous immunoassays. , 2005, Lab on a chip.

[22]  Ali Khademhosseini,et al.  Nano/Microfluidics for diagnosis of infectious diseases in developing countries. , 2010, Advanced drug delivery reviews.

[23]  Dafu Cui,et al.  Development of an integrated direct-contacting optical-fiber microchip with light-emitting diode-induced fluorescence detection. , 2007, Journal of chromatography. A.