Phytohaemagglutinin reactivity in circulating peripheral blood lymphocytes during a Trypanosoma brucei infection: sequential studies in individual guinea‐pigs

This study has analysed the response to phytohaemagglutinin of peripheral blood lymphocytes from guinea‐pigs infected with Trypanosoma brucei brucei. By this means it was possible to follow the response of individual animals throughout an infection. A culture method using whole blood permitted fewer cell manipulations and eliminated the necessity to supplement cultures with heterologous serum. Selection of appropriate strains of T.b. brucei produced a relatively chronic infection in guinea‐pigs. Results from this system indicate that, even late in the disease, significant mitogen reactivity still remains in some individuals. More significantly, these mitogen ‘responders’ controlled successive parasitaemic waves producing a fluctuating parasitaemia whereas the animals showing poor mitogen responsiveness—‘non‐responders’ failed to control successive waves and showed plateau parasitaemias.

[1]  F. Rurangirwa,et al.  Immunosuppression in bovine trypanosomiasis. The establishment of "memory" in cattle infected with T. congolense and the effect of post infection serum on in vitro (3H)-thymidine uptake by lymphocytes and on leucocyte migration. , 1980, Tropenmedizin und Parasitologie.

[2]  J. M. Mansfield,et al.  Lymphocyte function in experimental African trypanosomiasis. III. Loss of lymph node cell responsiveness. , 1980, Journal of immunology.

[3]  M. Selkirk,et al.  Intrinsic immunosuppressive activity of different trypanosome strains varies with parasite virulence , 1980, Nature.

[4]  R. Terry,et al.  Secondary immunodeficiencies associated with African trypanosomiasis. , 1980 .

[5]  G. Urquhart.  The pathogenesis and immunology of African trypanosomiasis in domestic animals. , 1980, Transactions of the Royal Society of Tropical Medicine and Hygiene.

[6]  G. Urquhart.  The effect of trypanosomiasis on the immunological apparatus [proceedings]. , 1980, Transactions of the Royal Society of Tropical Medicine and Hygiene.

[7]  R. Terry,et al.  Immunodepression and the course of infection of a chronic Trypanosoma brucei infection in mice , 1979, Parasite immunology.

[8]  C. Clayton,et al.  Trypanosoma brucei infection in nude mice: B lymphocyte function is suppressed in the absence of T lymphocytes , 1979, Parasite immunology.

[9]  J. M. Mansfield,et al.  Lymphocyte function in experimental African trypanosomiasis. II. Splenic suppressor cell activity. , 1979, Journal of immunology.

[10]  C. Clayton,et al.  Functional depletion of T- and B-memory cells and other lymphoid cell subpopulations-during trypanosomiasis. , 1979, Immunology.

[11]  B. Waksman,et al.  Activation of distinct helper and suppressor T cells in experimental trypanosomiasis. , 1978, Journal of immunology.

[12]  J. M. Mansfield.  Immunobiology of African trypanosomiasis. , 1978, Cellular immunology.

[13]  Taylor Dw,et al.  Effect of falciparum malaria infection on the in vitro mitogen responses of spleen and peripheral blood lymphocytes from owl monkeys. , 1978 .

[14]  D. Taylor,et al.  Effect of falciparum malaria infection on the in vitro mitogen responses of spleen and peripheral blood lymphocytes from owl monkeys. , 1978, The American journal of tropical medicine and hygiene.

[15]  D Franks,et al.  Statistics in cellular typing. , 2008, Tissue antigens.

[16]  B. Bo,et al.  Thymus dependency of Trypanosoma musculi elimination from mice. , 1977 .

[17]  A. Jayawardena,et al.  Suppressor cells in mice infected with Trypanosoma brucei. , 1977, Journal of immunology.

[18]  C. Clayton,et al.  Suppressor cells and loss of B-cell potential in mice infected with Trypanosoma brucei. , 1977, Clinical and experimental immunology.

[19]  R. Rank,et al.  Chronic infection with Trypanosoma musculi in congenitally athymic nude mice , 1977, Infection and immunity.

[20]  G. Targett,et al.  T lymphocytes and the transfer of immunity to Trypanosoma musculi in mice. , 1977, Clinical and experimental immunology.

[21]  B. Waksman,et al.  Suppressor cells in experimental trypanosomiasis , 1977, Nature.

[22]  B. O. Brooks,et al.  Thymus dependency of Trypanosoma musculi elimination from mice. , 1977, Journal of the Reticuloendothelial Society.

[23]  W. J. Herbert,et al.  Trypanosoma brucei: a rapid "matching" method for estimating the host's parasitemia. , 1976, Experimental parasitology.

[24]  J. Seed,et al.  Immunodepression during Trypanosoma brucei gambiense infections in the field vole, Microtus montanus. , 1976, Clinical and experimental immunology.

[25]  P. K. Murray,et al.  The nature of immunosuppression in Trypanosoma brucei infections in mice. II. The role of the T and B lymphocytes. , 1974, Immunology.

[26]  D. L. Moore,et al.  PHA-induced lymphocyte transformations in leucocyte cultures from malarious, malnourished and control Gambian children. , 1974, Clinical and experimental immunology.

[27]  J. Wallace,et al.  Suppression of Cell-Mediated Immunity in Experimental African Trypanosomiasis , 1974, Infection and immunity.

[28]  P. K. Murray,et al.  Immunosuppression in bovine trypanosomiasis , 1974, Veterinary Record.

[29]  P. K. Murray,et al.  Immunosuppression in Trypanosoma brucei infections in rats and mice. , 1973, Transactions of the Royal Society of Tropical Medicine and Hygiene.

[30]  P. K. Murray,et al.  The response of mice infected with T. brucei to the administration of sheep erythrocytes. , 1973, Transactions of the Royal Society of Tropical Medicine and Hygiene.

[31]  B. Greenwood,et al.  Immunosuppression in Gambian trypanosomiasis. , 1973, Transactions of the Royal Society of Tropical Medicine and Hygiene.

[32]  S. Reddy,et al.  Spontaneous lymphocyte transformation in leucocyte cultures of children with falciparum malaria. , 1972, Tropical and geographical medicine.

[33]  A. Bryceson,et al.  Immunosuppression in children with malaria. , 1972, Lancet.

[34]  G. Targett,et al.  Effect of Infection with Trypanosomes on the Development of Experimental Allergic Neuritis in Rabbits , 1971, Nature.

[35]  U. Junge,et al.  Microtechnique for quantitative evaluation of in vitro lymphocyte transformation. , 1970, Clinical and experimental immunology.

[36]  S. M. Lanham.  Separation of Trypanosomes from the Blood of Infected Rats and Mice by Anion-exchangers , 1968, Nature.

[37]  R. Killick-Kendrick,et al.  Cyclically transmitted infections of Trypanosoma brucei, T. rhodesiense and T. gambiense in chimpanzees. , 1967, Transactions of the Royal Society of Tropical Medicine and Hygiene.