The decline in energetic metabolism with aging of the erythrocyte and its relationship to cell death

Human erythrocytes were separated by buoyant density ultracentrifugation into fractions of progressively increasing mean cell age to measure the changes in glycolytic activity that occur during their 120‐day life‐span. The maximal activities of all glycolytic enzymes were shown to decline exponentially with cell age. Only three glycolytic enzymes exhibited a marked rate of decline with a t1/2 shorter than the cell life‐span: hexokinase, aldolase, and pyruvate kinase.

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