Complexation of HSA with different forms of antimony (Sb): An application of fluorescence spectroscopy

Abstract Antimony (Sb) pollution has been of a great environmental concern in some areas in China. Sb enters human body via drinking water, inhalation and food chain, unavoidably interacts with human serum albumin (HSA) in blood plasma, and consequently does harm to human health. The harmful effects of Sb on human health depend on the Sb species and their binding ability to HSA. In the present study, binding of three forms of Sb with HSA was investigated by excitation-emission matrix (EEM) spectroscopy. All of antimony potassium tartrate, antimony trichloride and potassium pyroantimonate quenched fluorescence of HSA. Values of conditional stability constant Ka (×105/M) for Sb and HSA systems were 8.13–9.12 for antimony potassium tartrate, 2.51–4.27 for antimony trichloride and 3.63–9.77 for potassium pyroantimonate. The binding constant Kb (×104/M) values of HSA with antimony potassium tartrate, antimony trichloride and potassium pyroantimonate were 0.02–0.07, 3.55–5.01, and 0.07–1.08, respectively. There was one independent class of binding site for antimony trichloride towards HSA. There was more than one Sb binding site and negative cooperativity between multiple binding sites for potassium pyroantimonate and antimony potassium tartrate towards HSA. The binding ability of HSA to complex Sb followed the order: antimony trichloride>potassium pyroantimonate>antimony potassium tartrate.

[1]  Guo‐Bo Li,et al.  Studies on the binding of rhaponticin with human serum albumin by molecular spectroscopy, modeling and equilibrium dialysis. , 2012, Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy.

[2]  L. Shao,et al.  Insight into the heavy metal binding potential of dissolved organic matter in MSW leachate using EEM quenching combined with PARAFAC analysis. , 2011, Water research.

[3]  N. Subbarao,et al.  Ligand binding strategies of human serum albumin: how can the cargo be utilized? , 2010, Chirality.

[4]  X. Yao,et al.  The binding of 3-(p-bromophenyl)-5-methyl-thiohydantoin with human serum albumin: Investigation by fluorescence spectroscopy and molecular model , 2007 .

[5]  C. Kuo,et al.  Antimony trichloride induces DNA damage and apoptosis in mammalian cells. , 1998, Toxicology.

[6]  Montserrat Filella,et al.  Antimony in the environment: a review focused on natural waters: I. Occurrence , 2002 .

[7]  Fei Ding,et al.  Complex of nicosulfuron with human serum albumin: A biophysical study , 2010 .

[8]  T. L. Hill,et al.  Cooperativity Theory in Biochemistry , 1985, Springer Series in Molecular and Cell Biology.

[9]  W. Maher.  Antimony in the environment – the new global puzzle , 2009 .

[10]  C. Garnier,et al.  Determinations of Uranium(VI) Binding Properties with some Metalloproteins (Transferrin, Albumin, Metallothionein and Ferritin) by Fluorescence Quenching , 2010, Journal of Fluorescence.

[11]  Rainer Schulin,et al.  Antimony in the soil-plant system - a review , 2009 .

[12]  X. Yao,et al.  Study of characterization and application on the binding between 5-iodouridine with HSA by spectroscopic and modeling , 2008 .

[13]  M. He,et al.  Effects of different forms of antimony on rice during the period of germination and growth and antimony concentration in rice tissue , 1999 .

[14]  R. H. Khan,et al.  Interaction studies of a novel Co(II)-based potential chemotherapeutic agent with human serum albumin (HSA) employing biophysical techniques , 2010, Medicinal Chemistry Research.

[15]  Zhide Hu,et al.  Binding studies of costunolide and dehydrocostuslactone with HSA by spectroscopy and atomic force microscopy , 2011 .

[16]  J. Lakowicz.  Principles of fluorescence spectroscopy , 1983 .

[17]  R. McClellan,et al.  Metabolism of two valence states of inhaled antimony in hamsters. , 1974, American Industrial Hygiene Association journal.

[18]  Patricia Smichowski,et al.  Antimony in the environment as a global pollutant: a review on analytical methodologies for its determination in atmospheric aerosols. , 2008, Talanta.

[19]  Jianlong Wang,et al.  Toxic effects of antimony on photosystem II of Synechocystis sp. as probed by in vivo chlorophyll fluorescence , 2010, Journal of Applied Phycology.

[20]  K. Booksh,et al.  Fluorescence excitation-emission matrix regional integration to quantify spectra for dissolved organic matter. , 2003, Environmental science & technology.

[21]  Ejaz Ahmad,et al.  Pollutant-Induced Modulation in Conformation and β-Lactamase Activity of Human Serum Albumin , 2012, PloS one.

[22]  Liang-Hong Guo,et al.  Fluorescence study on site-specific binding of perfluoroalkyl acids to human serum albumin , 2009, Archives of Toxicology.

[23]  F. Jiang,et al.  Binding of fullerol to human serum albumin: spectroscopic and electrochemical approach. , 2012, Journal of photochemistry and photobiology. B, Biology.

[24]  Yi Liu,et al.  Studies of interaction between colchicine and bovine serum albumin by fluorescence quenching method , 2005 .

[25]  Jimmy Flarakos,et al.  High-throughput solution-based medicinal library screening against human serum albumin. , 2005, Analytical chemistry.

[26]  M. Bozorgmehr,et al.  Investigation of the behavior of HSA upon binding to amlodipine and propranolol: Spectroscopic and molecular modeling approaches. , 2012, Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy.

[27]  Xi Chen,et al.  Antimony Accumulation, Growth Performance, Antioxidant Defense System and Photosynthesis of Zea mays in Response to Antimony Pollution in Soil , 2011 .

[28]  Ejaz Ahmad,et al.  Structural Stability as a Probe for Molecular Evolution of Homologous Albumins Studied by Spectroscopy and Bioinformatics , 2011, Cell Biochemistry and Biophysics.

[29]  D C Carter,et al.  Structure of serum albumin. , 1994, Advances in protein chemistry.

[30]  Yanqing Wang,et al.  Studies on the interaction between chromium(VI) and human serum albumin: spectroscopic approach. , 2012, Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy.

[31]  Cui Yanrui,et al.  Interaction of APT with BSA or HSA , 2006 .

[32]  Montserrat Filella,et al.  Antimony in the environment: a review focused on natural waters: II. Relevant solution chemistry , 2002 .

[33]  Rettig Pc.  ATSDR (Agency for Toxic Substances and Disease Registry): medical waste poses no threat to public. , 1990 .

[34]  Benyong Han,et al.  Study on the interaction between theasinesin and human serum albumin by fluorescence spectroscopy , 2010 .