THE EFFECTS OF ANTI-MITOTIC AGENTS ON DIHAPLOIDIZATION AND FERTILITY IN WINTER SQUASH (Cucurbita maxima Duch.) AND PUMPKIN (Cucurbita moschata Duch.) ANDROGENIC HAPLOIDS

Double-haploidization (DH) is one of the favorable techniques to obtain 100% pure double haploid plants (DH’s) for generating the new F1 cultivars in a short time. The fecundity of this technique depends on the high quantity of haploids and also fertile DH’s. However, there are no comprehensive reports on the chromosome doubling and fertility (fruit and seed-set) of winter squash and pumpkin haploids, currently. Thus, to obtain high frequency and fertile DH’s, the efficiency of different anti-mitotic agents (colchicine, amiprophos methyl, trifluralin and oryzalin) was tested at various concentrations and exposure times for both in vitro and in vivo conditions. Haploid plantlets recovered from anther cultures of winter squash and pumpkin lines were used for DH program. The haploid plants were wholly immersed in aqueous solutions of antimitotic agents in vitro and apical parts of haploid plantlets were treated with anti-mitotic agents three times in vivo. Since some plants remained haploid, and the highest DH efficiency was obtained from multiple treatments of colchicine to shoot tips of haploid plants in the rate of 93.3%. In vivo multiple treatments of 1% colchicine for an hour was found to be the best doubling procedure for the recovery of high-frequency fertile DH’s in our winter squash and pumpkin breeding program.

[1]  M. Aslam,et al.  In vivo Maternal Haploid Seed Production and Chromosome Doubling with different Anti-microtubular Agents in Maize , 2017 .

[2]  A. Balkaya,et al.  Evaluation of haploidization efficiency in winter squash (Cucurbita maxima Duch.) and pumpkin (Cucurbita moschata Duch.) through anther culture , 2016, Plant Cell, Tissue and Organ Culture (PCTOC).

[3]  Chen Peng-xiang,et al.  Effects of enhanced ultraviolet-B radiation on chromosomes and microtubule arrays in wheat roots. , 2016 .

[4]  R. Słomnicka,et al.  FROM POLLINATION TO DH LINES - VERIFICATION AND OPTIMIZATION OF PROTOCOL FOR PRODUCTION OF DOUBLED HAPLOIDS IN CUCUMBER , 2015 .

[5]  K. Niemirowicz-Szczytt,et al.  Diploidization of cucumber (Cucumis sativus L.) haploids by colchicine treatment , 2014 .

[6]  K. Niemirowicz-Szczytt,et al.  Review of research on haploid production in cucumber and other cucurbits , 2013 .

[7]  G. Kumar,et al.  Original Article Cytogenetical evidences of abnormal meiosis and 2n pollen formation via colchicine in microsporogenesis of Brassica campestris L. , 2013 .

[8]  E. Madadkhah,et al.  Production of haploid lines from parthenogenetic Iranian melon plants obtained of irradiated pollen ( Cucumis melo L.) , 2012 .

[9]  N. Sarı,et al.  Comparison of in vivo and in vitro Colchicine Application for Production of Dihaploid ‘Kirkagac’ and ‘Yuva Hasanbey’ Melons , 2011 .

[10]  K. Park,et al.  Effect of Different Pollination Dates with Irradiated Pollens on Fruit Set, Haploid Embryo Induction and Plant Obtention in Turkish (Kirkagac, Yuva and Hasanbey) Melons , 2010 .

[11]  A. Balkaya,et al.  Production of in vitro haploid plants from in situ induced haploid embryos in winter squash (Cucurbita maxima Duchesne ex Lam.) via irradiated pollen , 2010, Plant Cell, Tissue and Organ Culture (PCTOC).

[12]  Tenzile Ofluoglu,et al.  Induction of haploid embryo and plant regeneration via irradiated pollen technique in pumpkin (Cucurbita moschata Duchesne ex. Poir). , 2009 .

[13]  E. Earle,et al.  Enhanced recovery of doubled haploid lines from parthenogenetic plants of melon (Cucumis melo L.) , 2009, Plant Cell, Tissue and Organ Culture (PCTOC).

[14]  E. Earle,et al.  Effect of in vitro and in vivo colchicine treatments on pollen production and fruit set of melon plants obtained by pollination with irradiated pollen , 2008, Plant Cell, Tissue and Organ Culture.

[15]  M. Mutschler,et al.  Complementary strategies for ploidy manipulations in gynogenic onion (Allium cepa L.) , 2007 .

[16]  M. Germanà.  Doubled haploid production in fruit crops , 2006, Plant Cell, Tissue and Organ Culture.

[17]  J. Garcia-Mas,et al.  Optimization of Cucumber Doubled Haploid Line Production Using In Vitro Rescue of In Vivo Induced Parthenogenic Embryos , 2005 .

[18]  J. A. D. Silva,et al.  Meiose e viabilidade polínica na família Araceae , 2005 .

[19]  E. Grzebelus,et al.  Effect of anti-mitotic agents on development and genome doubling of gynogenic onion (Allium cepa L.) embryos , 2004 .

[20]  N. Sarı,et al.  Obtention of haploid embryos and plants through irradiated pollen technique in squash (Cucurbita pepo L.) , 2002, Euphytica.

[21]  N. Sarı,et al.  A new method for haploid muskmelon (Cucumis melo L.) dihaploidization , 2003 .

[22]  E. Earle,et al.  Production of haploid and doubled haploid plants of melon (Cucumis melo L.) for use in breeding for multiple virus resistance , 2003, Plant Cell Reports.

[23]  H. Ezura,et al.  EFFICIENT PRODUCTION OF DOUBLED HAPLOID MELON PLANTS BY MODIFIED COLCHICINE TREATMENT OF PARTHENOGENETIC HAPLOIDS , 2002 .

[24]  Nezihe Koksal,et al.  COMPARISON OF DIFFERENT IN VIVO METHODS FOR CHROMOSOME DUPLICATION IN MUSKMELON (CUCUMIS MELO) , 2002 .

[25]  A. Kashi,et al.  INDUCTION OF PARTHENOGENETIC EMBRYOS BY IRRADIATED POLLEN IN CUCUMBER , 1999 .

[26]  H. Horner,et al.  Mechanisms of genic male sterility , 1995 .

[27]  M. Nepi,et al.  Pollination, Pollen Viability and Pistil Receptivity in Cucurbita pepo , 1993 .