Title In Vivo Multiphoton Microscopy of Basal Cell Carcinoma

Importance—Basal cell carcinomas (BCCs) are diagnosed by clinical evaluation, which can include dermoscopic evaluation, biopsy, and histopathologic examination. Recent translation of Corresponding Author: Kristen M. Kelly, MD, Department of Dermatology, University of California–Irvine, 1002 Health Sciences Rd, Irvine, CA 92612 (kmkelly@uci.edu). Supplemental content at jamadermatology.com Author Contributions: Dr Balu had full access to all of the data in the study and takes responsibility for the integrity of the data and the accuracy of the data analysis. Study concept and design: Balu, Zachary, Krasieva, König, Tromberg, Kelly. Acquisition, analysis, or interpretation of data: Balu, Zachary, Harris, Krasieva, Tromberg, Kelly. Drafting of the manuscript: Balu, Zachary. Critical revision of the manuscript for important intellectual content: All authors. Statistical analysis: Harris. Obtained funding: Tromberg. Administrative, technical, or material support: Zachary, Krasieva, König, Tromberg. Study supervision: Zachary, Tromberg, Kelly. Conflict of Interest Disclosures: Dr König is cofounder of JenLab GmbH (Jena, Germany). Previous Presentation: This article was presented at the American Society for Laser Medicine & Surgery Annual Conference; April 24, Kissimmee, Florida. HHS Public Access Author manuscript JAMA Dermatol. Author manuscript; available in PMC 2016 October 01. Published in final edited form as: JAMA Dermatol. 2015 October 1; 151(10): 1068–1074. doi:10.1001/jamadermatol.2015.0453. A uhor M anscript

[1]  Mia K Markey,et al.  Clinical study of noninvasive in vivo melanoma and nonmelanoma skin cancers using multimodal spectral diagnosis , 2014, Journal of biomedical optics.

[2]  P. Gain,et al.  The role of in vivo confocal microscopy in the diagnosis of eyelid margin tumors: 47 cases. , 2014, Journal of the American Academy of Dermatology.

[3]  A. Lallas,et al.  Classifying distinct basal cell carcinoma subtype by means of dermatoscopy and reflectance confocal microscopy. , 2014, Journal of the American Academy of Dermatology.

[4]  T. Gambichler,et al.  Histopathological correlates of basal cell carcinoma in the slice and en face imaging modes of high‐definition optical coherence tomography , 2014, The British journal of dermatology.

[5]  S. González,et al.  Confocal microscopy patterns in nonmelanoma skin cancer and clinical applications. , 2014 .

[6]  Karsten König,et al.  In vivo detection of basal cell carcinoma: comparison of a reflectance confocal microscope and a multiphoton tomograph , 2013, Journal of biomedical optics.

[7]  Karsten König,et al.  High‐resolution imaging of basal cell carcinoma: a comparison between multiphoton microscopy with fluorescence lifetime imaging and reflectance confocal microscopy , 2013, Skin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging.

[8]  C. Berking,et al.  Morphology of basal cell carcinoma in high definition optical coherence tomography: en‐face and slice imaging mode, and comparison with histology , 2013, Journal of the European Academy of Dermatology and Venereology : JEADV.

[9]  C. Longo,et al.  In vivo confocal microscopy for diagnosis of melanoma and basal cell carcinoma using a two-step method: analysis of 710 consecutive clinically equivocal cases. , 2012, The Journal of investigative dermatology.

[10]  V. del Marmol,et al.  Imaging of basal cell carcinoma by high‐definition optical coherence tomography: histomorphological correlation. A pilot study , 2012, The British journal of dermatology.

[11]  K. König,et al.  Multiphoton Multispectral Fluorescence Lifetime Tomography for the Evaluation of Basal Cell Carcinomas , 2012, PloS one.

[12]  Maria Smedh,et al.  Multiphoton laser scanning microscopy on non-melanoma skin cancer: morphologic features for future non-invasive diagnostics. , 2008, The Journal of investigative dermatology.

[13]  Thilo Gambichler,et al.  In vivo optical coherence tomography of basal cell carcinoma. , 2007, Journal of dermatological science.

[14]  J. Schmitt,et al.  Optical coherence tomography for the characterization of basal cell carcinoma in vivo: a pilot study. , 2006, Journal of the American Academy of Dermatology.

[15]  Bruce J Tromberg,et al.  Effect of pulse duration on two-photon excited fluorescence and second harmonic generation in nonlinear optical microscopy. , 2006, Journal of biomedical optics.

[16]  S. González,et al.  Detection of Residual Basal Cell Carcinoma by In Vivo Confocal Microscopy , 2005, Dermatologic surgery : official publication for American Society for Dermatologic Surgery [et al.].

[17]  S. González,et al.  Sensitivity and specificity of reflectance-mode confocal microscopy for in vivo diagnosis of basal cell carcinoma: a multicenter study. , 2004, Journal of the American Academy of Dermatology.

[18]  Iris Riemann,et al.  High-resolution multiphoton tomography of human skin with subcellular spatial resolution and picosecond time resolution. , 2003, Journal of biomedical optics.

[19]  S. González,et al.  Real-time, in vivo confocal reflectance microscopy of basal cell carcinoma. , 2002, Journal of the American Academy of Dermatology.

[20]  P Altmeyer,et al.  Investigation of basal cell carcinoma [correction of carcionoma] by confocal laser scanning microscopy in vivo. , 2002, Skin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging.

[21]  D. Kuijpers,et al.  Basal Cell Carcinoma , 2002, American journal of clinical dermatology.

[22]  H. Pinkus EPITHELIAL AND FIBROEPITHELIAL TUMORS. , 1965, Bulletin of the New York Academy of Medicine.

[23]  E F TRAUB,et al.  Cancer of skin. , 1954, Bulletin. New York Medical College.

[24]  W. F. Lever,et al.  Histopathology of the Skin , 1962 .