ANTHOCYANIN1 of Petunia Controls Pigment Synthesis, Vacuolar pH, and Seed Coat Development by Genetically Distinct Mechanisms Article, publication date, and citation information can be found at www.plantcell.org/cgi/doi/10.1105/tpc.003772.

ANTHOCYANIN1 (AN1) of petunia is a transcription factor of the basic helix-loop-helix (bHLH) family that is required for the synthesis of anthocyanin pigments. Here, we show that AN1 controls additional aspects of cell differentiation: the acidification of vacuoles in petal cells, and the size and morphology of cells in the seed coat epidermis. We identified an1 alleles, formerly known as ph6, that sustain anthocyanin synthesis but not vacuolar acidification and seed coat morphogenesis. These alleles express truncated proteins lacking the C-terminal half of AN1, including the bHLH domain, at an ∼30-fold higher level than wild-type AN1. An allelic series in which one, two, or three amino acids were inserted into the bHLH domain indicated that this domain is required for both anthocyanin synthesis and vacuolar acidification. These findings show that AN1 controls more aspects of epidermal cell differentiation than previously thought through partially separable domains.

[1]  W. Koomen,et al.  Genetic analysis of instability in Petunia hybrida , 1984, Theoretical and Applied Genetics.

[2]  A. Schram,et al.  Genes affecting flower colour and pH of flower limb homogenates in Petunia hybrida , 1983, Theoretical and Applied Genetics.

[3]  F. Bianchi,et al.  Regulation of gene action in Petunia hybrida: Unstable alleles of a gene for flower colour , 1978, Theoretical and Applied Genetics.

[4]  B. Weisshaar,et al.  A. thaliana TRANSPARENT TESTA 1 is involved in seed coat development and defines the WIP subfamily of plant zinc finger proteins. , 2002, Genes & development.

[5]  L. Lepiniec,et al.  The Arabidopsis TT2 Gene Encodes an R2R3 MYB Domain Protein That Acts as a Key Determinant for Proanthocyanidin Accumulation in Developing Seed , 2001, The Plant Cell Online.

[6]  B. Winkel-Shirley,et al.  Flavonoid biosynthesis. A colorful model for genetics, biochemistry, cell biology, and biotechnology. , 2001, Plant physiology.

[7]  J. Schiefelbein,et al.  Developmentally distinct MYB genes encode functionally equivalent proteins in Arabidopsis. , 2001, Development.

[8]  A. Peeters,et al.  The TRANSPARENT TESTA12 Gene of Arabidopsis Encodes a Multidrug Secondary Transporter-like Protein Required for Flavonoid Sequestration in Vacuoles of the Seed Coat Endothelium , 2001, Plant Cell.

[9]  Richard A. Dixon,et al.  Activation Tagging Identifies a Conserved MYB Regulator of Phenylpropanoid Biosynthesis , 2000, Plant Cell.

[10]  Fan Zhang,et al.  GL3 encodes a bHLH protein that regulates trichome development in arabidopsis through interaction with GL1 and TTG1. , 2000, Genetics.

[11]  S. Iida,et al.  Colour-enhancing protein in blue petals , 2000, Nature.

[12]  L. Lepiniec,et al.  The TT8 Gene Encodes a Basic Helix-Loop-Helix Domain Protein Required for Expression of DFR and BAN Genes in Arabidopsis Siliques , 2000, Plant Cell.

[13]  C Spelt,et al.  anthocyanin1 of Petunia Encodes a Basic Helix-Loop-Helix Protein That Directly Activates Transcription of Structural Anthocyanin Genes , 2000, Plant Cell.

[14]  J. Mendenhall,et al.  Arabidopsis seed coat development: morphological differentiation of the outer integument. , 2000, The Plant journal : for cell and molecular biology.

[15]  D. Szymanski,et al.  Progress in the molecular genetic analysis of trichome initiation and morphogenesis in Arabidopsis. , 2000, Trends in plant science.

[16]  M. Koornneef,et al.  Influence of the testa on seed dormancy, germination, and longevity in Arabidopsis. , 2000, Plant physiology.

[17]  G. Haughn,et al.  Differentiation of mucilage secretory cells of the Arabidopsis seed coat. , 2000, Plant physiology.

[18]  C. Murre,et al.  Helix-Loop-Helix Proteins: Regulators of Transcription in Eucaryotic Organisms , 2000, Molecular and Cellular Biology.

[19]  O. Folkerts,et al.  Expression Profiling of the Maize Flavonoid Pathway Genes Controlled by Estradiol-Inducible Transcription Factors CRC and P , 2000, Plant Cell.

[20]  T. Payne,et al.  GL 3 Encodes a bHLH Protein That Regulates Trichome Development in Arabidopsis Through Interaction With GL 1 and TTG 1 C . , 2000 .

[21]  J. Schiefelbein,et al.  WEREWOLF, a MYB-Related Protein in Arabidopsis, Is a Position-Dependent Regulator of Epidermal Cell Patterning , 1999, Cell.

[22]  J. Mol,et al.  Molecular Analysis of the anthocyanin2 Gene of Petunia and Its Role in the Evolution of Flower Color , 1999, Plant Cell.

[23]  C. James,et al.  The TRANSPARENT TESTA GLABRA1 Locus, Which Regulates Trichome Differentiation and Anthocyanin Biosynthesis in Arabidopsis, Encodes a WD40 Repeat Protein , 1999, Plant Cell.

[24]  M Koornneef,et al.  ANTHOCYANINLESS2, a Homeobox Gene Affecting Anthocyanin Distribution and Root Development in Arabidopsis , 1999, Plant Cell.

[25]  D. Arnold,et al.  Heterologous myb genes distinct from GL1 enhance trichome production when overexpressed in Nicotiana tabacum. , 1999, Development.

[26]  S. Wessler,et al.  Molecular consequences of Ds insertion into and excision from the helix-loop-helix domain of the maize R gene. , 1998, Genetics.

[27]  V. Walbot,et al.  Functional Complementation of Anthocyanin Sequestration in the Vacuole by Widely Divergent Glutathione S-Transferases , 1998, Plant Cell.

[28]  E. Grotewold,et al.  How genes paint flowers and seeds , 1998 .

[29]  J. Mol,et al.  Analysis of bHLH and MYB domain proteins: species-specific regulatory differences are caused by divergent evolution of target anthocyanin genes. , 1998, The Plant journal : for cell and molecular biology.

[30]  A. van Houwelingen,et al.  Analysis of flower pigmentation mutants generated by random transposon mutagenesis in Petunia hybrida. , 1998, The Plant journal : for cell and molecular biology.

[31]  P. A. Rea,et al.  AtMRP1 gene of Arabidopsis encodes a glutathione S-conjugate pump: isolation and functional definition of a plant ATP-binding cassette transporter gene. , 1997, Proceedings of the National Academy of Sciences of the United States of America.

[32]  J. Mol,et al.  The an11 locus controlling flower pigmentation in petunia encodes a novel WD-repeat protein conserved in yeast, plants, and animals. , 1997, Genes & development.

[33]  P. Wittich,et al.  Downregulation of ovule-specific MADS box genes from petunia results in maternally controlled defects in seed development. , 1997, The Plant cell.

[34]  J. Mol,et al.  The No Apical Meristem Gene of Petunia Is Required for Pattern Formation in Embryos and Flowers and Is Expressed at Meristem and Primordia Boundaries , 1996, Cell.

[35]  T. Holton,et al.  Genetics and Biochemistry of Anthocyanin Biosynthesis. , 1995, The Plant cell.

[36]  Y. Okazaki,et al.  Cause of blue petal colour , 1995, Nature.

[37]  Ronald W. Davis,et al.  The TTG gene is required to specify epidermal cell fate and cell patterning in the Arabidopsis root. , 1994, Developmental biology.

[38]  R. W. Davis,et al.  Epidermal cell fate determination in Arabidopsis: patterns defined by a steroid-inducible regulator. , 1994, Science.

[39]  H. Huits,et al.  Genetic control of dihydroflavonol 4-reductase gene expression in Petunia hybrida. , 1994, The Plant journal : for cell and molecular biology.

[40]  J. Mol,et al.  The flavonoid biosynthetic pathway in plants: Function and evolution , 1994 .

[41]  J. Mol,et al.  Regulatory Genes Controlling Anthocyanin Pigmentation Are Functionally Conserved among Plant Species and Have Distinct Sets of Target Genes. , 1993, The Plant cell.

[42]  E. Ralston,et al.  Tagging and Cloning of a Petunia Flower Color Gene with the Maize Transposable Element Activator. , 1993, The Plant cell.

[43]  Ronald W. Davis,et al.  Arabidopsis and Nicotiana anthocyanin production activated by maize regulators R and C1. , 1992, Science.

[44]  Taiz.  THE PLANT VACUOLE. , 1992, The Journal of experimental biology.

[45]  S. Goff,et al.  Functional analysis of the transcriptional activator encoded by the maize B gene: evidence for a direct functional interaction between two classes of regulatory proteins. , 1992, Genes & development.

[46]  R. Koes,et al.  Chalcone Synthase Promoters in Petunia Are Active in Pigmented and Unpigmented Cell Types. , 1990, The Plant cell.

[47]  M. Frohman,et al.  Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer. , 1988, Proceedings of the National Academy of Sciences of the United States of America.