Experimental allergic arthritis in the rabbit: alterations in the cellularity and the rate of cellular proliferation in the synovial linings of the challenged joints of rabbits immunized with antigen in Freund's incomplete adjuvant.

Rabbits have been immunized with ovalbumin in Freund's incomplete adjuvant (FIA) followed by the intra-articular injection of ovalbumin, in order to follow the development of inflammation in the synovial lining. The kinetics of cell proliferation have been investigated using tritiated thymidine (3HTdR) autoradiography and Feulgen cytophotometry. Unexpectedly, marked histological changes were found in the synovium, with hyperplasia of synoviocytes, and of the connective-tissue cells of the subintima, being seen as early as 3 days after challenge. Large numbers of inflammatory cells, including many plasma cells, were found in the synovium at between 5 and 11 days. Labelling of synoviocytes and connective-tissue cells reached a maximum at 3 days and declined thereafter, reaching normal levels at 14 days. Three weeks after challenge the synovium was normal in appearance.

[1]  B. Henderson,et al.  Evidence for cell division in synoviocytes in acutely inflamed rabbit joints. , 1981, Annals of the rheumatic diseases.

[2]  E. Lederer,et al.  Enhancement of nonspecific immunity to Klebsiella pneumoniae infection by a synthetic immunoadjuvant (N-acetylmuramyl-L-alanyl-D-isoglutamine) and several analogs. , 1977, Proceedings of the National Academy of Sciences of the United States of America.

[3]  L. Glynn,et al.  Is persisting antigen responsible for the chronicity of experimental allergic arthritis? , 1977, Annals of the rheumatic diseases.

[4]  A. Doble,et al.  The non-passage of mycobacteria from Freund's complete adjuvant granuloma depots to arthritic joints. , 1975, British journal of experimental pathology.

[5]  L. Glynn,et al.  Persistence of antigen in nonarthritic joints. , 1975, Annals of the rheumatic diseases.

[6]  A. Cohen,et al.  Fine structural analysis of rabbit synovial cells. I. The normal synovium and changes in organ culture. , 1973, Arthritis and rheumatism.

[7]  T. Cooke,et al.  THE PATHOGENESIS OF CHRONIC INFLAMMATION IN EXPERIMENTAL ANTIGEN-INDUCED ARTHRITIS , 1972, The Journal of experimental medicine.

[8]  A. Doble,et al.  Production of a chronic arthritis with ovalbumin. Its retention in the rabbit knee joint. , 1971, Annals of the rheumatic diseases.

[9]  L. Glynn,et al.  The production of arthritis in rabbits by an immunological reaction to fibrin. , 1962, British journal of experimental pathology.

[10]  P. Gell,et al.  Observations on the histology of the Arthus reaction and its relation to other known types of skin hypersensitivity. , 1954, International archives of allergy and applied immunology.