Expression of NGF receptor in the developing and adult primate central nervous system

Monoclonal antibodies against human NGF receptor have been used for immunocytochemical localization of NGF receptors in the CNS of macaques and baboons at various stages of development. In the adult, neurons in most brain regions are devoid of detectable NGF receptors. However, abundant NGF receptor immunoreactivity is present on a population of neurons in basal forebrain, which, on the basis of appearance and pattern of distribution, probably correspond, at least in part, to magnocellular cholinergic neurons of this region. NGF receptors were also associated with the vasculature in most brain regions. NGF receptor immunoreactivity is present on Mueller glia of neural retina. In macaque fetuses, approximately 1 month prenatally, retinal Mueller glia possess lower levels of receptor, while higher levels of receptor are present in the retinal nerve fiber layer. In fetal cerebellum, abundant receptor immunoreactivity is present on Purkinje cells, granule cells of the premigratory zone of the external granule layer, and neurons of the deep nuclei. Immunoreactivity decreases with subsequent development and is absent in the adult. In cerebellum, levels of NGF receptor assayed by affinity crosslinking to radioiodinated NGF, and levels of NGF receptor mRNA assayed by Northern blot analysis decrease dramatically during the last month of fetal life.

[1]  M. Schwab,et al.  Specific retrograde transport of nerve growth factor (NGF) from neocortex to nucleus basalis in the rat , 1984, Brain Research.

[2]  P. Grob,et al.  Characterization of the human melanoma nerve growth factor receptor. , 1985, The Journal of biological chemistry.

[3]  D. Mash,et al.  Localization of nerve growth factor receptors in cholinergic neurons of the human basal forebrain , 1986, Neuroscience Letters.

[4]  W. Rutter,et al.  Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. , 1979, Biochemistry.

[5]  P. Thomas,et al.  Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. , 1980, Proceedings of the National Academy of Sciences of the United States of America.

[6]  U. K. Laemmli,et al.  Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 , 1970, Nature.

[7]  P Berg,et al.  Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. , 1977, Journal of molecular biology.

[8]  M. Johnston,et al.  Choline acetyltransferase activity in striatum of neonatal rats increased by nerve growth factor. , 1985, Science.

[9]  M. Chao,et al.  Expression and structure of the human NGF receptor , 1986, Cell.

[10]  H. Boedtker,et al.  RNA molecular weight determinations by gel electrophoresis under denaturing conditions, a critical reexamination. , 1977, Biochemistry.

[11]  P. Grob,et al.  Purification and Amino Terminal Sequencing of Human Melanoma Nerve Growth Factor Receptor , 1987, Journal of neurochemistry.

[12]  H. Thoenen,et al.  Levels of nerve growth factor and its mRNA in the central nervous system of the rat correlate with cholinergic innervation. , 1985, The EMBO journal.

[13]  R. Riopelle,et al.  Distribution of neuronal receptors for nerve growth factor in the rat , 1986, The Journal of neuroscience : the official journal of the Society for Neuroscience.

[14]  M. Bothwell,et al.  Nerve growth factor receptors on PC12 cells: Evidence for two receptor classes with differing cytoskeletal association , 1981, Cell.

[15]  E. Shooter,et al.  A monoclonal antibody modulates the interaction of nerve growth factor with PC12 cells. , 1984, The Journal of biological chemistry.

[16]  G. Weskamp,et al.  Nerve growth factor gene expression in the developing rat brain. , 1986, Science.

[17]  I. Black,et al.  Nerve growth factor promotes cholinergic development in brain striatal cultures. , 1985, Proceedings of the National Academy of Sciences of the United States of America.

[18]  M. Schwab,et al.  NGF-mediated increase of choline acetyltransferase (ChAT) in the neonatal rat forebrain: evidence for a physiological role of NGF in the brain? , 1983, Brain research.

[19]  L. Reichardt,et al.  Expression of the beta-nerve growth factor gene correlates with the density of sympathetic innervation in effector organs. , 1984, Proceedings of the National Academy of Sciences of the United States of America.

[20]  A. Winfree,et al.  Postnatal development of the cerebellar cortex in the rat: V. Spatial organization of Purkinje cell perikarya , 1977, The Journal of comparative neurology.

[21]  D. Helfman,et al.  Co-existence of vinculin and a vinculin-like protein of higher molecular weight in smooth muscle. , 1982, The Journal of biological chemistry.

[22]  G. Edelman,et al.  Nerve growth factor enhances expression of neuron-glia cell adhesion molecule in PC12 cells , 1986, The Journal of cell biology.

[23]  E. Bock,et al.  Demonstration of immunochemical identity between the nerve growth factor‐inducible large external (NILE) glycoprotein and the cell adhesion molecule L1. , 1985, The EMBO journal.

[24]  J. Schweitzer,et al.  Nerve growth factor receptor molecules in rat brain. , 1986, Proceedings of the National Academy of Sciences of the United States of America.

[25]  P. Honegger,et al.  Nerve growth factor (NGF) stimulation of cholinergic telencephalic neurons in aggregating cell cultures. , 1982, Brain research.

[26]  J. Pontén,et al.  The ultrastructure of human and murine astrocytes and of human fibroblasts in culture. , 2009, Acta pathologica et microbiologica Scandinavica. Section A, Pathology.

[27]  D. Elder,et al.  Characterization of nerve growth factor receptor in neural crest tumors using monoclonal antibodies. , 1984, Proceedings of the National Academy of Sciences of the United States of America.

[28]  I. Verma,et al.  Induction of the proto-oncogene fos by nerve growth factor. , 1985, Proceedings of the National Academy of Sciences of the United States of America.

[29]  L. Reichardt,et al.  Studies on the expression of the beta nerve growth factor (NGF) gene in the central nervous system: level and regional distribution of NGF mRNA suggest that NGF functions as a trophic factor for several distinct populations of neurons. , 1986, Proceedings of the National Academy of Sciences of the United States of America.

[30]  J. Turner,et al.  Regenerative repair in the severed optic nerve of the newt (Triturus viridescens): Effect of nerve growth factor , 1977, Experimental Neurology.

[31]  M. Schwab,et al.  Nerve growth factor increases choline acetyl-transferase but not survival or fiber outgrowth of cultured fetal septal cholinergic neurons , 1985, Neuroscience.

[32]  J. Sedmak,et al.  A rapid, sensitive, and versatile assay for protein using Coomassie brilliant blue G250. , 1977, Analytical biochemistry.

[33]  G. Edelman,et al.  Sequential expression and differential function of multiple adhesion molecules during the formation of cerebellar cortical layers , 1987, The Journal of cell biology.