Microdynamics of the piezo-driven pipettes in ICSI

Undesirably low success rates have been reported in the intracytoplasmic sperm injection procedure. Recently a method using piezo-driven pipettes with a very small mercury column contributed substantial improvements in this process. Despite the toxicity of mercury, this new procedure is commonly utilized in many laboratories. However, there is no study available to date on the micromechanics of this procedure. The underlying principles of piercing are not clear for both cases, with and without the mercury. Presently, the pressure burst, which is caused by the abrupt axial motion of the mercury column, is attributed to this effect. Here, we take the mercury-filled pipettes and try to understand the governing physics. The findings point out the occurrence of considerable lateral tip oscillations of the injection pipette as the piezoelectric pulse train is introduced. We claim that the lateral dynamics play an important role in the piercing and should be considered to enlighten the process and the effects of the mercury. These claims are analytically studied and experimentally verified.

[1]  R Yanagimachi,et al.  Intracytoplasmic sperm injection experiments using the mouse as a model. , 1998, Human reproduction.

[2]  P. Devroey,et al.  Pregnancies after intracytoplasmic injection of single spermatozoon into an oocyte , 1992, The Lancet.

[3]  Michelle Plachot,et al.  Outcome of conventional IVF and ICSI on sibling oocytes in mild male factor infertility. , 2002, Human reproduction.

[4]  K. Jishage,et al.  Effect of Partial Incision of the Zona Pellucida by Piezo-Micromanipulator for In Vitro Fertilization Using Frozen-Thawed Mouse Spermatozoa on the Developmental Rate of Embryos Transferred at the 2-Cell Stage , 2002, Biology of reproduction.

[5]  R Yanagimachi,et al.  Intracytoplasmic sperm injection in the rat , 1998, Zygote.

[6]  M. Derde,et al.  Neonatal data on a cohort of 2889 infants born after ICSI (1991-1999) and of 2995 infants born after IVF (1983-1999). , 2002, Human reproduction.

[7]  H. Katayose,et al.  The usefulness of a piezo-micromanipulator in intracytoplasmic sperm injection in humans. , 1999, Human reproduction.

[8]  R Yanagimachi,et al.  Comparison of intracytoplasmic sperm injection for inbred and hybrid mice , 2001, Molecular reproduction and development.

[9]  R Yanagimachi,et al.  Analysis of mouse oocyte activation suggests the involvement of sperm perinuclear material. , 1998, Biology of reproduction.

[10]  M. Stojkovic,et al.  Intracytoplasmic sperm injection in bovine: effects of oocyte activation, sperm pretreatment and injection technique. , 2000, Theriogenology.

[11]  H. Fujiwara,et al.  A new assisted hatching technique using a piezo-micromanipulator. , 1998, Fertility and sterility.

[12]  Shigeto Takeuchi,et al.  Comparison of Piezo-Assisted Micromanipulation with Conventional Micromanipulation for Intracytoplasmic Sperm Injection into Human Oocytes , 2001, Gynecologic and Obstetric Investigation.

[13]  Z. Nagy,et al.  Intracytoplasmic sperm injection in the mouse. , 1995, Human reproduction.

[14]  Thomas T. F. Huang,et al.  The use of piezo micromanipulation for intracytoplasmic sperm injection of human oocytes , 1996, Journal of Assisted Reproduction and Genetics.

[15]  Alan Trounson,et al.  Development of enucleated bovine oocytes , 1999 .

[16]  A Alosilla Fonttis,et al.  Successful ICSI in a case of severe asthenozoospermia due to 93% non-specific axonemal alterations and 90% abnormal or absent mitochondrial sheaths. , 2002, Reproductive biomedicine online.

[17]  P. Collas,et al.  Nuclear transplantation by microinjection of inner cell mass and granulosa cell nuclei , 1994, Molecular reproduction and development.

[18]  Teruhiko Wakayama,et al.  Development of normal mice from oocytes injected with freeze-dried spermatozoa , 1998, Nature Biotechnology.

[19]  T. Kawahara,et al.  Efficient injection of bull spermatozoa into oocytes using a Piezo-driven pipette. , 1999, Theriogenology.

[20]  D. Wolf,et al.  Sperm-induced oocyte activation in the rhesus monkey: nuclear and cytoplasmic changes following intracytoplasmic sperm injection. , 1997, Human reproduction.