Preliminary tests of maximum likelihood image reconstruction method on 3-D real data and some practical considerations for the data corrections

The image reconstruction method of maximum likelihood estimation (MLE) has been used in the authors' previous work for fluorescence microscopy. By computer simulations, it was previously found that this method worked very well for addressing two dimensional superresolution and three dimensional optical sectioning problems. In this paper, the results of 3D reconstructions with real data will be presented.