Immunocytochemical Localization of Prunasin Hydrolase and Mandelonitrile Lyase in Stems and Leaves of Prunus serotina

In macerates of black cherry (Prunus serotina Ehrh.) leaves and stems, (R)-prunasin is catabolized to HCN, benzaldehyde, and D-glucose by the sequential action of prunasin hydrolase (EC 3.2.1.21) and (R)-(+)-mandelonitrile lyase (EC 4.1.2.10). Immuno-cytochemical techniques have shown that within these organs prunasin hydrolase occurs within the vacuoles of phloem parenchyma cells. In arborescent leaves, mandelonitrile lyase was also located in phloem parenchyma vacuoles, but comparison of serial sections revealed that these two degradative enzymes are usually localized within different cells.

[1]  J. Poulton,et al.  Utilization of Amygdalin during Seedling Development of Prunus serotina , 1994, Plant physiology.

[2]  J. Poulton,et al.  Tissue Level Compartmentation of (R)-Amygdalin and Amygdalin Hydrolase Prevents Large-Scale Cyanogenesis in Undamaged Prunus Seeds , 1994, Plant physiology.

[3]  J. Poulton.  Enzymology of cyanogenesis in rosaceous stone fruits , 1993 .

[4]  K. Palme,et al.  A protein from maize labeled with azido‐IAA has novel β‐glucosidase activity , 1992 .

[5]  A. Hughes.  In-situ localization of cyanogenic β-glucosidase (linamarase) gene expression in leaves of cassava (Manihot esculenta Cranz) using non-isotopic riboprobes , 1992 .

[6]  J. Poulton,et al.  Tissue and Subcellular Localization of Enzymes Catabolizing (R)-Amygdalin in Mature Prunus serotina Seeds. , 1992, Plant physiology.

[7]  J. Poulton,et al.  Prunus serotina Amygdalin Hydrolase and Prunasin Hydrolase : Purification, N-Terminal Sequencing, and Antibody Production. , 1992, Plant physiology.

[8]  J. Poulton,et al.  Development of the Potential for Cyanogenesis in Maturing Black Cherry (Prunus serotina Ehrh.) Fruits. , 1992, Plant physiology.

[9]  J. Poulton,et al.  Immunocytochemical Localization of Mandelonitrile Lyase in Mature Black Cherry (Prunus serotina Ehrh.) Seeds. , 1991, Plant physiology.

[10]  D. Jones.  Cyanogenesis in animal-plant interactions. , 2007, Ciba Foundation symposium.

[11]  D. Selmar,et al.  Mobilization and utilization of cyanogenic glycosides: the linustatin pathway. , 1988, Plant physiology.

[12]  J. Poulton.  Localization and catabolism of cyanogenic glycosides. , 1988, Ciba Foundation symposium.

[13]  J. Poulton,et al.  Tissue Distributions of Dhurrin and of Enzymes Involved in Its Metabolism in Leaves of Sorghum bicolor. , 1979, Plant physiology.

[14]  John Merriam Kingsbury,et al.  Poisonous plants of the United States and Canada , 1964 .